针刺研究

2013, v.38(05) 358-364

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针刺对快速老化P8亚系小鼠海马神经元丢失与星形胶质细胞增生的干预作用
Effect of Acupuncture Intervention on Hippocampal Neuron Loss and Astrocytosis in SAMP 8 Mice

张月峰;于建春;张雪竹;韩景献;
ZHANG Yue-feng;YU Jian-chun;ZHANG Xue-zhu;HAN Jing-xian;Department of Acupuncture and Moxibustion of the Hospital of Liaocheng University;First Teaching Hospital of Tianjin University of Chinese Medicine;

摘要(Abstract):

目的:观察针刺对快速老化模型小鼠P 8亚系(SAMP 8)海马神经元丢失与星形胶质细胞(AC)增生的干预作用,为针刺治疗阿尔茨海默病(AD)提供实验依据。方法:18只雄性SAMP 8小鼠随机分为SAMP 8模型组、针刺组、非穴组,每组6只;6只雄性快速老化模型小鼠R 1系(SAMR 1)作为SAMR 1对照组。针刺组采用"三焦针法",取"膻中""中脘""气海""足三里""血海"穴,每日1次,共14d;非穴组选双胁下髂嵴垂直向上10、15mm各两个固定非穴点。治疗结束后,制备脑切片,分别作甲苯胺蓝染色和免疫组化染色,观察海马神经元形态和数量及胶质原纤维酸性蛋白(GFAP)的表达情况。结果:与对照组相比,其它3组海马神经元均有不同程度丢失(P<0.01),针刺组神经元丢失减少(P<0.05);模型组海马GFAP表达多于对照组(P<0.01),针刺组GFAP表达较模型组明显减少(P<0.01);GFAP表达与神经元数量呈显著负相关(P<0.01)。结论:"三焦针法"可以减少或延迟SAMP 8小鼠神经元的丢失,减少星形胶质细胞的活化增生,调整神经元与星形胶质细胞关系,维持了中枢神经系统的正常结构和功能,对AD的治疗可能具有积极的作用。
Objective To observe the effect of"Sanjiao Acupuncture Therapy"on hippocampal selective neuron loss and astrocytosis in senile accelerated mice(SAM)so as to reveal its mechanism underlying improvement of Alzheimer's disease(AD).Methods A total of 18seven-month old male SAM prone 8(SAMP 8)were selected and randomly allocated into model group,acupuncture group,and non-acupoint group.Moreover,6age-and sex-matched SAM resistant 1(SAMR 1)were enrolled as the homology normal control group.For mice of the acupuncture group,"Sanjiao Acupuncture Therapy"[manual acupuncture stimulation of"Danzhong"(CV 17),"Zhongwan"(CV 12),"Qihai"(CV 6),"Zusanli"(ST 36)and"Xuehai"(SP 10)]was given,and for rats of the non-acupoint group,manual acupuncture stimulation was applied to bilateral costal region(non-acupoints,below the costal region and 10and 15mm superior to the iliac crest).The treatment was conducted once daily for 14days.At the end of each experiment,the mouse's hippocampus tissue was taken out after routine perfusion and fixing for paraffin sections which were stained by toluidine blue technique for observing changes of neurons and by immunohistochemistry for analyzing glial fibrillary acidic protein(GFAP)immunoactivity of astrocytes.Results Compared with the control group,a marked neuron loss was found in the model,acupuncture and non-acupoint groups(P<0.01).The numbers of neurons of hippocampal CA 1and CA 3regions in the acupuncture group were significantly more than those in the model group(P<0.05).Compared with the control group,the expression level of hippocampal GFAP was markedly increased in the model group(P<0.01).In comparison with the control group,the average expression area,optical density values and the number of GFAP-labeled astocytes were significantly upregulated in the model group(P<0.01).While in comparison with the model group,these 3indexes were apparently downregulated in the acupuncture group(P<0.01)but not in the non-acupoint group except the average expression area,suggesting a reduction of hippocampal astrocyte activity after acupuncture intervention.A negative correlation was found between the expression level of GFAP and the number of astrocytes.Conclusion "Sanjiao Acupuncture Therapy"can lower or delay neuron loss,suppress astrocytosis,and regulate the relationship between neuron and astrocyte,which may contribute to its function in relieving AD.

关键词(KeyWords): 阿尔茨海默病;针刺疗法;神经元丢失;胶质原纤维酸性蛋白表达;星形胶质细胞;快速老化小鼠
AD(Alzheimer's disease);Acupuncture therapy;Neuron loss;Glial fibrillary acidic protein expression;Astrocyte;Senile accelerated mouse(SAM

Abstract:

Keywords:

基金项目(Foundation): 天津市应用基础与前沿技术研究计划重点项目(11JCZDJC 20100);; 高等学校博士学科点专项科研基金(20101210110005);; 国家自然科学青年基金(30901927);; 天津市应用基础及前沿技术研究计划(09JCYBJC 12600)

作者(Author): 张月峰;于建春;张雪竹;韩景献;
ZHANG Yue-feng;YU Jian-chun;ZHANG Xue-zhu;HAN Jing-xian;Department of Acupuncture and Moxibustion of the Hospital of Liaocheng University;First Teaching Hospital of Tianjin University of Chinese Medicine;

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DOI: 10.13702/j.1000-0607.2013.05.003

参考文献(References):

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