张慧;何胜东;纵单单;张雪梅;罗婧;郑佳坤;

• 1:西南医科大学中西医结合学院
• 2:桂林医学院第二附属医院
• 3:中南大学湘雅二医院
• 4:北京中医药大学研究生院

摘要(Abstract)：

目的:观察电针对偏头痛大鼠三叉神经节miR-34a-5p、沉默信息调节因子1(SIRT1)及核因子-κB亚基p65(NF-κB p65)表达的影响,探讨电针预防偏头痛的作用机制。方法:雄性SD大鼠随机分为正常组、假手术组、模型组、电针组及电针+EX527(SIRT1抑制剂)组,每组10只。电刺激三叉神经节建立偏头痛大鼠模型。电针组、电针+EX527组大鼠建模前连续5 d分别给予电针"外关""风池"干预,每次20 min,1次/d,电针+EX527组同时每天腹腔注射EX527(5 mg/kg)。建模后第1天处死大鼠,酶联免疫吸附法检测大鼠血清前列腺素E2(PGE2)和降钙素基因相关肽(CGRP)的浓度,实时荧光定量PCR法检测三叉神经节miR-34a-5p及SIRT1、白细胞介素-1β(IL-1β)的mRNA水平,Western blot法检测三叉神经节SIRT1、IL-1β、 NF-κB p65、乙酰化NF-κB p65(NF-κB Ac-p65)和环氧化酶2(COX2)蛋白表达。结果:与正常组和假手术组比较,模型组大鼠血清PGE2和CGRP浓度升高(P<0.05),三叉神经节miR-34a-5p表达增加(P<0.05)、 SIRT1 mRNA和蛋白表达减少(P<0.05)、IL-1βmRNA和蛋白表达增加(P<0.05),NF-κB p65、NF-κB Ac-p65和COX2蛋白表达增加(P<0.05);与模型组比较,电针组血清PGE2和CGRP浓度降低(P<0.05),三叉神经节miR-34a-5p表达减少(P<0.05)、SIRT1 mRNA和蛋白表达增加(P<0.05)、IL-1βmRNA和蛋白表达减少(P<0.05)、NF-κB p65、NF-κB Ac-p65和COX2蛋白表达减少(P<0.05);与电针组比较,电针+EX527组血清PGE2和CGRP浓度升高(P<0.05),三叉神经节IL-1βmRNA和蛋白表达增加(P<0.05),SIRT1蛋白表达减少(P<0.05),NF-κB p65、NF-κB Ac-p65和COX2蛋白表达增加(P<0.05)。结论:电针可抑制偏头痛大鼠三叉神经节miR-34a-5p表达,增加SIRT1表达,下调IL-1β/COX2炎性反应信号,减少PGE2合成,进而减少三叉神经末梢释放CGRP,这可能是电针预防偏头痛的机制之一。

关键词(KeyWords)： 偏头痛;电针;三叉神经节;miR-34a-5p;沉默信息调节因子1;核因子-κB亚基p65

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(No.81704173);; 四川省教育厅重点项目(No.17ZA0662)

作者(Author): 张慧;何胜东;纵单单;张雪梅;罗婧;郑佳坤;

Email:

DOI: 10.13702/j.1000-0607.200378

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