针刺研究

2015, v.40(01) 30-34+55

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电针“百会”“涌泉”对APP/PS 1双转基因小鼠海马β淀粉样蛋白及低密度脂蛋白受体相关蛋白-1水平的影响
Effect of Electroacupuncture Stimulation of“Baihui”(GV 20)and“Yongquan”(KI 1)on Expression of Hippocampal Amyloid-β and Low Density Lipoprotein Receptor-related Protein-1 in APP/PS 1 Transgenic Mice

李芙;李丽娜;王鑫;白杨;加吾拉·阿不力孜;步青云;高堂珂;薛卫国;
LI Fu;LI Li-na;WANG Xin;BAI Yang;JIAWULA·Abulizi;BU Qing-yun;GAO Tang-ke;XUE Wei-guo;School of Acu-moxibustion and Tuina,Beijing University of Chinese Medicine;School of Basic Medicine,Beijing University of Chinese Medicine;

摘要(Abstract):

目的:观察电针对APP/PS 1转基因小鼠β淀粉样蛋白(Aβ)及其清除途径的关键转运受体低密度脂蛋白受体相关蛋白-1(LRP 1)的可能影响,探讨电针干预阿尔茨海默病的可能途径。方法 :将4月龄APP/PS 1双转基因小鼠随机分为模型组、电针治疗组,以背景小鼠C 57BL/6为正常对照组。电针治疗组予电针"涌泉""百会",15min/次,隔日1次,治疗6周。采用Morris水迷宫测试小鼠空间记忆行为学,ELISA法检测海马Aβ1-40、Aβ1-42含量,Western blot法检测海马LRP 1表达。结果:Morris水迷宫测试显示,各组定位航行实验逃避潜伏时以及空间探索实验穿越平台象限次数、平台象限停留时长的差异没有统计学意义(P>0.05),模型组逃避潜伏时相比正常对照组有延长趋势,而电针治疗组逃避潜伏时较模型组有缩短趋势。模型组海马Aβ1-42、Aβ1-40含量及Aβ1-42/Aβ1-40值均显著高于正常对照组(P<0.01),电针治疗组Aβ1-42、Aβ1-40含量及Aβ1-42/Aβ1-40值均显著低于模型组(P<0.01)。模型组海马LRP 1蛋白相对表达量低于正常对照组(P<0.05),电针治疗组LRP 1相对表达量与模型组比较差异无统计学意义(P>0.05)。结论:电针可降低APP/PS 1双转基因小鼠海马Aβ水平,其机制是否与Aβ转运受体LRP 1相关尚需进一步探讨。
Objective To observe the effect of electroacupuncture(EA)treatment on the level of hippocampal amyloidbeta peptide(Aβ)and its key transport receptor low density lipoprotein receptor-related protein-1(LRP 1)in APP/PS 1transgenic mice so as to explore its mechanism underlying improvement of Alzheimer's disease(AD).Methods Twenty-four male APP/PS 1transgenic mice were equally and randomly divided into model group and EA treatment group,and 12C57BL/6mice were used as the normal control group.EA(1Hz/50 Hz,0.3mA)was applied to"Baihui"(GV 20)and"Yongquan"(KI 1)for15min,once every other day for 6weeks.The learning-memory ability was detected by using Morris water maze testing,left hippocampal Aβ1-40 and Aβ1-42 contents were assayed by ELISA,and right hippocampal LRP 1expression was detected using Western blot(WB).Results Results of Morris water maze test showed no significant differences among the three groups in the escape latency,the times of the platform-site crossovers,the time spent in the target platform quadrant(P>0.05).Compared with the model group,the moderately increased escape latency had a decreasing tendency in the EA treatment group.ELISA assaying showed that hippocampal Aβ1-42,Aβ1-40,and ratio of Aβ1-42/Aβ1-40 of the model group were significantly higher than those of the normal control group(P<0.01).After EA intervention,the increased Aβ1-42,Aβ1-40,and ratio of Aβ1-42/Aβ1-40 were remarkably down-regulated in the EA treatment group(P<0.01).WB detection displayed that the right hippocampal LRP 1expression level of the model group was markedly lower than that of the normal control group(P<0.05).After EA treatment,LRP 1expression level was moderately up-regulated but without significant difference between the model and EA treatment groups(P>0.05).Conclusion EA intervention can lower the level of hippocampal Aβin APP/PS 1transgenic mice,but its effects on Aβtransport receptor LRP 1expression and learning-memory ability need being confirmed further.

关键词(KeyWords): 电针;阿尔茨海默病;β淀粉样蛋白;低密度脂蛋白受体相关蛋白-1;海马
Electroacupuncture;Alzheimer's disease;Amyloidβ;Low density lipoprotein receptor-related protein-1;Hippocampus

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.81273826);; 北京中医药大学自主选题(No.2014-JYBZZ-XS-044)

作者(Author): 李芙;李丽娜;王鑫;白杨;加吾拉·阿不力孜;步青云;高堂珂;薛卫国;
LI Fu;LI Li-na;WANG Xin;BAI Yang;JIAWULA·Abulizi;BU Qing-yun;GAO Tang-ke;XUE Wei-guo;School of Acu-moxibustion and Tuina,Beijing University of Chinese Medicine;School of Basic Medicine,Beijing University of Chinese Medicine;

Email:

DOI: 10.13702/j.1000-0607.2015.01.006

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