针刺研究

2021, v.46(01) 58-63

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电针预处理对急性心肌缺血大鼠心肌瞬时受体电位亚型1/降钙素基因相关肽信号及核因子-κB亚型p65蛋白表达的影响
Effect of electroacupuncture pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP) signal and NF-κB p65 protein expression in rats with acute myocardial ischemia

吴嘉宏;孙珂;卢圣锋;白桦;徐森磊;夏雪峰;刘雨晨;顾一煌;张宏如;
WU Jia-hong;SUN Ke;LU Sheng-feng;BAI Hua;XU Sen-lei;XIA Xue-feng;LIU Yu-chen;GU Yi-huang;ZHANG Hong-ru;College of Acupuncture and Massage,Health and Rehabilitation College,Nanjing University of Chinese Medicine;Key Laboratory for Researching Combination of Acupuncture and Drugs of Ministry of Education,Nanjing University of Chinese Medicine;

摘要(Abstract):

目的:观察电针预处理对急性心肌缺血(AMI)损伤大鼠心肌组织瞬时受体电位亚型1(TRPV1)/降钙素基因相关肽(CGRP)信号及核因子-κB亚型p65(NF-κB p65)蛋白表达的影响,探讨电针预处理抗AMI损伤的可能机制。方法:SD大鼠随机分为空白组、假手术组、模型组和电针组,每组15只。模型组和电针组采用冠状动脉左前降支结扎术建立AMI大鼠模型。电针组选取双侧"内关"穴进行电针干预,每次20 min,每日1次,造膜前连续干预7 d。采用生理信号采集系统记录心电图(ECG),观察术前、术后30 min、术后24 h标准肢体Ⅱ导联ST段电位偏移值情况;TTC染色观察心肌梗死面积百分比;HE染色观察心肌组织病理形态变化及炎性细胞浸润程度;Western blot法检测心肌组织TRPV1/CGRP信号和NF-κB p65蛋白表达水平。结果:与假手术组比较,模型组大鼠术后30 min ECG-J点电位明显升高(P<0.05),术后24 hECG-J点电位明显降低(P<0.05),心肌梗死面积显著增加(P<0.05),心肌纤维紊乱,炎性细胞浸润明显,心肌TRPV1、CGRP及NF-κB p65蛋白表达均升高(P<0.05)。与模型组比较,电针组大鼠术后30 min ECG-J点电位明显下降(P<0.05),心肌梗死面积显著减少(P<0.05),心肌纤维形态改善,炎性细胞浸润减少,心肌TRPV1、CGRP蛋白表达升高(P<0.05),NF-κB p65蛋白表达降低(P<0.05)。结论:电针预处理可能通过加强心肌TRPV1/CGRP信号,下调NF-κB p65蛋白表达,降低心肌炎性反应状态,改善大鼠AMI损伤,减少心肌梗死面积。
Objective To observe the effect of electroacupuncture(EA)pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP)signal and nuclear factor-κB p65(NF-κB p65) protein expression in myocardial tissue of acute myocardial ischemic injury(AMI) rats, and to investigate the possible mechanism of electroacupuncture pretreatment against AMI. Methods A total of 60 adult male SD rats were randomly divided into blank control, sham operation, model and EA pretreatment groups, 15 rats in each group. The acute myocardial ischemia model was established by ligating the left anterior descending(LAD)branch of the coronary artery in the model group and EA pretreatment group, while threading but no ligating at left anterior descending branch of the coronary artery was applied in the sham operation group. In the EA pretreatment group, bilateral "Neiguan"(PC6) acupoints were selected, with intensity of 2 mA and frequency of 2 Hz/100 Hz, for 20 min, once daily for 7 days before modeling. Electrocardiogram(ECG) was recorded by physiological signal acquisition system, and the ST segment potential offset values of standard Ⅱ lead were analyzed before surgery,30 min and 24 h after operation. The TTC staining was used to observe the percentage of myocardial infarction area. The HE staining was used to observe the pathological changes of myocardial tissue and the degree of inflammatory cell infiltration. And Western blot was used to detect TRPV1/CGRP signal and NF-κB p65 protein expression levels in myocardial tissue. Results Compared with the sham operation group, the ECG-J point potential in the model group was significantly increased at 30 min and decreased at 24 h after operation(P<0.05), myocardial infarction area increased significantly(P<0.05), the myocardial fibers were obviously disordered, inflammatory cell infiltration was obvious, and the expressions of TRPV1,CGRP and NF-κB p65 proteins were all increased(P<0.05). Compared with the model group, the EA pretreatment group was decreased in the ECG-J point potential at 30 min after operation(P<0.05), significantly reduced in myocardial infarction area(P<0.05), improved in the morphology of myocardial fibers, reduced ininflammatory cell infiltration, and increased in the protein expressions of TRPV1 and CGRP in myocardium(P<0.05), significantly decreased in the protein expression of NF-κB p65(P<0.05). Conclusion EA pretreatment may enhance TRPV1/CGRP signaling, down-regulate NF-κB p65 protein expression, reduce myocardial inflammatory response status, improve AMI injury, and reduce myocardial infarction area.

关键词(KeyWords): 急性心肌缺血;电针预处理;瞬时受体电位香草酸亚型1/降钙素基因相关肽;核因子-κB亚型p 65
Acute myocardial ischemia;Electroacupuncture pretreatment;Transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP);Nuclear factor-κB p65(NF-κB p65)

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(No.81674063、81974583);国家自然科学基金青年项目(No.81704169);; 江苏省中医药领军人才项目(No.SLJ0226);; 江苏省自然科学基金青年项目(No.BK20171067)

作者(Author): 吴嘉宏;孙珂;卢圣锋;白桦;徐森磊;夏雪峰;刘雨晨;顾一煌;张宏如;
WU Jia-hong;SUN Ke;LU Sheng-feng;BAI Hua;XU Sen-lei;XIA Xue-feng;LIU Yu-chen;GU Yi-huang;ZHANG Hong-ru;College of Acupuncture and Massage,Health and Rehabilitation College,Nanjing University of Chinese Medicine;Key Laboratory for Researching Combination of Acupuncture and Drugs of Ministry of Education,Nanjing University of Chinese Medicine;

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DOI: 10.13702/j.1000-0607.200905

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