针刺研究

2021, v.46(09) 769-774

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艾灸对肌筋膜疼痛综合征大鼠脊髓背角小胶质细胞和脑源性神经营养因子表达的影响
Effect of moxibustion on the expression of microglia and brain-derived neurotrophic factor protein in the spinal cord of rats with myofascial pain syndrome

马俊杰;马铁明;王列;胡哲;
MA Jun-jie;MA Tie-ming;WANG Lie;HU Zhe;School of Acupuncture and Moxibustion, Liaoning University of Traditional Chinese Medicine;Liaoning Province Key Laboratory of Acupuncture and Health Rehabilitation;

摘要(Abstract):

目的:观察艾灸激痛点对肌筋膜疼痛综合征(MPS)大鼠脊髓小胶质细胞及脑源性神经营养因子(BDNF)表达的影响,探讨艾灸镇痛效应的部分中枢机制。方法:SD大鼠随机分为空白组、模型组、艾灸组,每组8只。采用钝性打击联合离心运动法制备MPS大鼠模型。艾灸组大鼠左股内侧肌激痛点部位行温和灸,每日1次,每次15 min,治疗7 d。分别于造模前及治疗前后1 d,使用热刺痛仪检测热痛阈值(TWL);HE染色法观察大鼠股内侧肌病理形态变化;免疫组织化学法检测大鼠脊髓背角小胶质细胞标记物OX-42表达;蛋白免疫印迹法检测大鼠脊髓BDNF蛋白表达。结果:造模后,模型组、艾灸组大鼠TWL较本组造模前及同时点空白组显著降低(P<0.01);治疗后艾灸组大鼠TWL较本组造模后及同时点模型组显著升高(P<0.01)。空白组肌纤维粗细均匀,排列紧密而规律;模型组见部分肌纤维断裂和结缔结构组织新生,排列不规则、紊乱,炎性细胞浸润;艾灸组肌纤维形态接近正常,排列较整齐有序,少量炎性细胞浸润。与空白组比较,模型组大鼠脊髓背角OX-42染色变深、数量增多、胞体肥大,平均吸光度值升高(P<0.01),经治疗后艾灸组OX-42染色变浅、数量减少、胞体变小,平均吸光度值降低(P<0.05)。与空白组比较,模型组大鼠脊髓BDNF蛋白表达上调(P<0.01);与模型组比较,艾灸组BDNF蛋白表达下调(P<0.05)。结论:艾灸可以明显改善MPS大鼠股内侧肌组织损伤,提高热痛阈值,其部分中枢机制可能与抑制脊髓背角小胶质细胞活化,降低BDNF蛋白表达有关。
Objective To observe the effect of moxibustion at myofascial trigger points on microglia activation and the expression of brain-derived neurotrophic factor(BDNF) in the spinal cord of rats with myofascial pain syndrome(MPS), so as to explore the central mechanism of the analgesic effect of moxibustion. Methods Twenty-four male SD rats were randomly divi-ded into control, model and moxibustion groups(n=8 in each group). The MPS model was established by strinking on fascia musculares and eccentric exercise. Rats in the moxibustion group were treated with mild moxibustion at myofascial trigger point for 15 min, once daily for 7 days. The thermal withdrawal latency(TWL) of rats was measured with a hot stabbing instrument. The pathological changes of the rat medial femoris muscle were observed after H.E. staining. The expressions of microglia marker(OX-42) and BDNF in the spinal dorsal horn were detected by immunohistochemistry and Western blot, separately. Results After modeling, the TWL of both the model and the moxibustion groups were significantly down-regulated(P<0.01),and were significantly decreased in contrast to that of the control group(P<0.01). After treatment and compared with the model group, the TWL of the moxibustion group was significantly increased(P<0.01) in the moxibustion group. Rat's muscle fibers of the control group were uniform in thickness and arranged tightly and regularly. While in the model group, some fractures and connective structure tissue renewal, irregular arrangement, and inflammatory cell infiltration were seen. The morphology of muscle fibers in the moxibustion group was close to normal, and the arrangement was neat and orderly, with a small amount of inflammatory cell infiltration. Compared with the control group, the expression of OX-42 and BDNF in the spinal dorsal horn of rats in the model group was increased(P<0.01). Following the treatment, and in comparison with the model group, the expression of OX-42 and BDNF of moxibustion group was down-regulated(P<0.05). Conclusion Moxibustion can significantly improve the injury of the medial femoral muscle and the TWL in MPS rats, which may be related to its effects in inhibiting the activation of spinal dorsal horn microglia and reducing the expression of BDNF.

关键词(KeyWords): 肌筋膜疼痛综合征;艾灸;激痛点;小胶质细胞;脑源性神经营养因子
Myofascial pain syndrome;Moxibustion;Myofascial trigger points;Microglia;Brain-derived neurotrophic factor

Abstract:

Keywords:

基金项目(Foundation): 辽宁省自然科学基金项目(No.2019ZD0947);; 辽宁省“兴辽英才计划”项目(No.XLYC1902004)

作者(Author): 马俊杰;马铁明;王列;胡哲;
MA Jun-jie;MA Tie-ming;WANG Lie;HU Zhe;School of Acupuncture and Moxibustion, Liaoning University of Traditional Chinese Medicine;Liaoning Province Key Laboratory of Acupuncture and Health Rehabilitation;

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DOI: 10.13702/j.1000-0607.20210222

参考文献(References):

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