针刺研究

2015, v.40(04) 270-274

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“益肾调督”针灸法对阿尔茨海默病大鼠海马神经元线粒体动力学相关蛋白的影响
Effect of“Kidney-reinforcing and Govenor Vessel-regulating”of Acupuncture plus Moxibustion on Mitochondrial Dynamics-related Proteins in Hippocampal Neurons of Rats with Alzheimer's Disease

罗磊;孙国杰;杜艳军;
LUO Lei;SUN Guo-jie;DU Yan-jun;School of Acu-moxibustion and Tuina,Henan University of Traditional Chinese Medicine;College of Acupuncture and Traumatology and Orthopedics,Hubei University of Traditional Chinese Medicine;

摘要(Abstract):

目的:观察"益肾调督"针灸法对阿尔茨海默病模型大鼠海马神经元线粒体动力学相关蛋白,即线粒体分裂蛋白1(Fis 1)和线粒体融合蛋白1(OPA 1)的影响,探讨其在保护线粒体过程中的可能有效机制。方法:将Wistar大鼠随机分为正常组、假手术组、模型组和针灸治疗组,每组20只。运用海马注射Aβ1-42的方法复制模型。针灸治疗组采用针刺加灸"百会"和"肾俞"穴的方法治疗,每日1次,每次15min,7d为1个疗程,共计2个疗程,疗程间休息1d。运用蛋白印迹技术和免疫荧光技术检测海马Fis 1和OPA 1的水平。结果:模型组海马神经元OPA 1的水平明显低于正常组和假手术组(P<0.01),Fis 1水平明显高于正常组和假手术组(P<0.01);而"益肾调督"针灸法能够有效上调OPA 1的水平,下调Fis 1的水平,与模型组比较差异具有统计学意义(P<0.01)。结论:"益肾调督"针灸法可能通过调节线粒体分裂与融合蛋白的失衡,改善线粒体动力学异常,从而达到改善线粒体损伤,保护线粒体功能的目的。
Objective To observe the effect of acupuncture plus moxibustion on mitochondrial dynamics-related proteins(OPA 1,Fis 1)of hippocampal neurons in rat with Alzheimer's disease(AD),and to explore the potential mechanism underlying acupuncture for the protection of hippocampal neuronal mitochondria.Methods Eighty male Wistar rats were randomly divided into normal,sham-operation,model and acu-moxibustion groups.AD model was made by injecting Aβ1-42 into the hippocampus."Baihui"(GV 20)and"Shenshu"(BL 23)acupoints were stimulated by manual acupuncture(15min)in combination with moxibustion(15min)for two sessions of treatment,with seven consecutive treatments for one session and one day break between the two sessions.The expression of hippocampal OPA 1and Fis 1was assessed by means of Western blot and immunofluorescence technique.Results The expression level of OPA 1and Fis 1of rats in the model group was significantly lower and higher,respectively,than those of the normal and sham-operation groups(P<0.01).Acupuncture plus moxibustion intervention notably increased OPA 1and decreased Fis 1expression levels in the acu-moxibustion group compared to those of the model group(P<0.01).Conclusion Acupuncture combined with moxibustion produce benefit to hippocampal neurons in AD rats probably via the regulation of the mitochondrial dynamics(imbalance between fission and fusion proteins)and improvement of the mitochondrial damage of hippocampus.

关键词(KeyWords): 益肾调督;针灸;阿尔茨海默病;线粒体分裂蛋白1;线粒体融合蛋白1
Kidney-reinforcement and Govenor Vessel-regulation;Acu-moxibustion;Alzheimer's disease;Fis 1;OPA 1

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.81273864,No.81173323)

作者(Author): 罗磊;孙国杰;杜艳军;
LUO Lei;SUN Guo-jie;DU Yan-jun;School of Acu-moxibustion and Tuina,Henan University of Traditional Chinese Medicine;College of Acupuncture and Traumatology and Orthopedics,Hubei University of Traditional Chinese Medicine;

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DOI: 10.13702/j.1000-0607.2015.04.003

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