针刺研究

1999, (04) 274-277

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电针下调大鼠局灶性脑缺血/再灌注时大脑皮质促凋亡基因bax蛋白表达
Electroacupuncture Can Downregulate Apoptotic Gene Bax Protein Expression in Cerebral Cortex during Focal Cerebral Ischemia/Reperfusion in Rats

罗勇,董为伟
Luo Yong, Dong Weiwei (Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 )

摘要(Abstract):

线栓法闭塞 Wistar大鼠大脑中动脉,制备局灶性脑缺血/再灌注模型。运用免疫组化法观测电针双侧“合谷”穴(LI4)区对局灶性脑缺血/再灌注时大脑皮质促凋亡基因bax蛋白的表达。结果显示,假手术组大脑皮质bax蛋白弱表达,局灶性脑缺血3小时大脑皮质bax蛋白表达增加,但未达显著水平( P> 0. 05),再灌注 3小时、 6小时大脑皮质 bax蛋白表达明显增加( P<0. 01),而电针可以明显减少再灌注 3小时、6小时大脑皮质 bax蛋白表达( P< 0. 01)。结果提示,电针“合谷”穴( LI4)区可下调大脑皮质促凋亡基因 bax蛋白的表达,这可能与电针抗局灶性脑缺血及再灌注时脑细胞凋亡有关。
The model of reversible middle cerebral artery (MCA) ischemia/reperfusion was established by suture method in Wistar rats. Immunohistochemistry method was uesd to detect the apoptotic gene bax protein expression in cerebral cortex. The effect of electroacupuncture (EA) on apoptotic gene bax pro tein expression in cerebral cortex was evaluated in the focal cerebral ischemia/reperfusion Wistar rats, including control group, focal cerebral ischemia/reperfusion groups and electroacupuncture groups. In control group bax protein expression was weakly positive. In I3h group bax protein expression increased partly (P>0. 05), bax protein expression increased markedly in I3h/R3h group and I3h/R6h group (P<0.01 ), and EA could make the bax protein expression decrease significantly in I3h/R3h group and I3h/R6h group (P < 0. 01 ). These results suggested that the antiapoptotic mechanism of EA at bilateral "Hegu" Points (LI4) may be related to the downregulation of bax protein expression.

关键词(KeyWords): 局灶性脑缺血;再灌注;凋亡;bax蛋白;电针;“合谷”穴

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金!(No.39730170);; 重庆市中医管理局资助课题

作者(Author): 罗勇,董为伟
Luo Yong, Dong Weiwei (Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 )

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