针刺研究

2006, (05) 259-263+271+256

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艾灸对应激性胃溃疡大鼠胃粘膜细胞增殖和凋亡的影响及其与热休克蛋白表达关系的研究
Effects of Moxibustion on Proliferation and Apoptosis of Gastric Mucosal Cells and Their Relationship with Heat Shock Protein Expression in Stress Gastric Ulcer Rats

易受乡;彭艳;常小荣;彭娜;严洁;林亚平;
YI Shou-xiang,PENG Yan,CHANG Xiao-rong,PENG Na,YAN Jie,LIN Ya-ping (Department of Acu-moxibustion and Massage,Hunan College of Chinese Medicine,Changsha 410007)

摘要(Abstract):

目的:观察艾灸“足三里”等穴对应激性胃溃疡大鼠胃粘膜细胞增殖及凋亡的影响,分析热休克蛋白70基因表达(HSP70 mRNA)与上述效应的关系,探讨艾灸促进胃粘膜损伤修复的细胞分子生物学机制。方法:60只健康SD大鼠随机分为4组,即束缚对照组、模型组、艾灸“足三里”-“梁门”穴组和艾灸非穴对照点组。束缚水浸应激法制备胃溃疡模型,采用放射免疫方法测定胃粘膜转化生长因子(TGFα-)的含量,RT-PCR法测定HSP70 mRNA,免疫组织化学方法检测胃粘膜增殖细胞核抗原(PCNA)及细胞凋亡指数。结果:束缚水浸应激法造模后可致胃粘膜损伤指数升高,胃粘膜TGFα-含量下降,PCNA下降,细胞凋亡指数、HSP70 mRNA增加(P<0.05或P<0.01)。艾灸“足三里”“梁门”可降低胃粘膜损伤指数,增加胃粘膜TGFα-含量,促进HSP70 mRNA和PCNA的表达,降低胃粘膜细胞凋亡指数,与模型组和对照组比较有显著性差异(P<0.05或P<0.01)。结论:艾灸“足三里”“梁门”对应激性胃溃疡的胃粘膜具有保护作用,其机制可能是促进了TGFα-合成,刺激胃粘膜细胞增殖,抑制细胞凋亡,而这一过程可能与艾灸诱导HSP70表达有关。
Objective: To observe the effect of moxibustion at "Zusanli"(ST 36)-"Liangmen"(ST 21) on proliferation and apoptosis of gastric mucosal cells and the relationship between the effect of moxibustion and the expression of heat shock protein 70(HSP70) mRNA so as to explore the underlying molecular biological mechanism of moxibustion in accelerating the repair of the injured gastric mucosa. Methods: Sixty SD rats were randomly assigned to control,model,moxibustion-ST 36+ST 21 and moxibustion non-acupoint groups with 15 cases in each group.Gastric ulcer model was established by fasting(24 h) and forced water(20 ℃) immersion(10 h).Moxibustion was applied to unilateral ST 36,ST 21 or non-acupoint(about 1 cm lateral to ST 21 and ST 36) for about 30 min(4 cones/point),once daily and continuously for 8 days.At the end of the experiments,the rat's gastric mucosa was sampled for examining gastromucosal injury index(ulcer index,UI,GUTH's method),apoptosis index(apoptosis reagent kit),proliferating cell nuclear antigen(PCNA,immunohistochemical method) activity,transforming growth factor α(TGF-α) content(radioimmunoassay),and HSP70 mRNA expression(reversed transcription-polymerase chain reaction,RT-PCR) separately. Results: Compared with control group,gastric mucosal UI,apoptosis index and HSP70 mRNA expression of model group increased significantly(P<0.01,0.05),while TGF-α content and PCNA numerical density decreased remarkably(P<0.01,0.05).While in comparison with model group,UI and apoptosis index of ST 36+ST 21 group lowered considerably(P<0.01,0.05),and TGF-α level,PCNA numerical density,and HSP70 mRNA expression of ST 36+ST 21 group increased remarkably(P<0.01).No significant differences were found between model and non-acupoint groups in UI,TGF-α leveland apoptosis index,and between ST 36+ST 21 and non-acupoint groups in PCNA numerical density(P>0.05). Conclusion: Moxibustion at "Zusanli"(ST 36)+"Liangmen"(ST 21) has a protective effect on gastromucosa in stress gastric ulcer rats,which is closely related to its actions in promoting synthesis of TGF-α and proliferation of gastromucosal cells,suppressing gastromucosal cell apoptosis,and upregulating HSP70 mRNA expression.

关键词(KeyWords): 应激性胃溃疡;艾灸;HSP70mRNA;细胞凋亡;PCNA
Stress gastric ulcer Moxibustion HSP70 mRNA Cell apoptosis PCNA

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.30572310);; 湖南省自然科学基金(05JJ4008)资助

作者(Author): 易受乡;彭艳;常小荣;彭娜;严洁;林亚平;
YI Shou-xiang,PENG Yan,CHANG Xiao-rong,PENG Na,YAN Jie,LIN Ya-ping (Department of Acu-moxibustion and Massage,Hunan College of Chinese Medicine,Changsha 410007)

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