针刺研究

2011, v.36(02) 90-94

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“嗅三针”对阿尔茨海默病大鼠海马毒覃碱型受体的影响
Effect of "Xiusanzhen" on Hippocampal Muscarinic Cholinergic Receptor Activity in Alzheimer Disease Rats

杨晓航;刘智斌;牛文民;牛晓梅;王渊;
YANG Xiao-hang,LIU Zhi-bin,NIU Wen-min,NIU Xiao-mei,WANG Yuan(Department of Medical Technology,Shaanxi College of Chinese Medicine,Xianyang 712046,China)

摘要(Abstract):

目的:探讨"嗅三针"对阿尔茨海默病(AD)模型大鼠海马胆碱能毒覃碱型受体(M受体)表达及功能的影响,了解其对嗅觉通路完整性的依赖作用。方法:成年SD雄性大鼠40只,随机分为正常对照组、AD模型组、嗅神经切断+嗅三针组和嗅三针组,每组10只。采用Aβ1-40淀粉样肽注射法制作AD大鼠模型,嗅神经切断术造大鼠嗅觉通路阻断模型,取"印堂"穴及两侧"迎香"穴交替电针刺激行"嗅三针"治疗。采用放射配体的单点结合分析法及Lowry微量定蛋白法测定海马组织胆碱能M受体最大结合量(Bmax)、平衡解离常数(Kd)及受体密度。结果:与正常对照组比较,AD模型组及嗅神经切断+嗅三针组大鼠M受体Bmax和密度显著降低(P<0.05);与AD模型组和嗅神经切断+嗅三针组比较,嗅三针组大鼠M受体Bmax和密度显著升高(P<0.05)。Kd的结果与之相反,与正常对照组比较,AD模型组及嗅神经切断+嗅三针组大鼠Kd显著提高(P<0.05);嗅三针组大鼠Kd与AD模型组和嗅神经切断+嗅三针组比较显著降低(P<0.05)。结论:"嗅三针"能够显著提高海马组织的胆碱能M受体Bmax和密度,降低Kd,其干预效应依赖于嗅觉传导通路的完整性。
Objective To explore the effect of "Xiusanzhen" [electroacupuncture(EA) of bilateral "Yingxiang"(LI20) and "Yintang"(EX-HN3)] on activities of hipppocampal muscarinic cholinergic receptor(mAChR) and the involved neural path in Alzheimer Disease(AD)rats.Methods Forty Sprague Dawley rats were randomly divided into normal control,model,olfactory nerve severing(ONS)-EA of "Xiusanzhen"(ONS-EA) and EA of "Xiusanzhen"(EA) groups.AD model was established by intra-hippocampal injection(AP:3.5mm,L:2mm,H:-2.8mm) of Aβ1-40 starch-like peptide(10 μg/2μL) under the aid of a microsyringe installed in a brain stereotaxis instrument.For rats of the ONS-EA group,the olfactory nerve was severed by using a surgical knife after drilling a hole on the skull(5mm anterior to the bregma,L,R: 2mm).The mAChR density,and its maximum binding capacity(Bmax) and dissociation constant(Kd) of the hippocampus tissue were measured by using radio-ligand binding analysis and Lowry's microamount protein assay.Results In comparison with the normal control group,the hippocampal mAChR density and its Bmax in the model group were decreased remarkably(P<0.05),while the Kd of M-receptor in the model group was increased remarkably(P<0.05).In comparison with the model group,hippocampal mAChR density and its Bmax in the EA group were up-regulated obviously(P<0.05),while the Kd of mAChR in the EA group was down-regulated significantly(P<0.05).No significant differences were found between the model and ONS-EA groups in mAChR density and its Bmax and Kd(P>0.05).Conclusion "Xiusanzhen"-EA can effectively up-regulate hippocampal mAChR density and Bmax and down-regulate Kd of M-receptor of hippocampus in AD rats,which is dependent on the intact olfactory nerve pathway.

关键词(KeyWords): 阿尔茨海默病;嗅三针;电针;胆碱能M受体;海马
Alzheimer Disease;Xiusanzhen;Electroacupuncture;Muscarinic cholinergic receptor;Hippocampus

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助(No.30973792)

作者(Author): 杨晓航;刘智斌;牛文民;牛晓梅;王渊;
YANG Xiao-hang,LIU Zhi-bin,NIU Wen-min,NIU Xiao-mei,WANG Yuan(Department of Medical Technology,Shaanxi College of Chinese Medicine,Xianyang 712046,China)

Email:

DOI: 10.13702/j.1000-0607.2011.02.004

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