针刺研究

2011, v.36(03) 171-175

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电针“天枢”穴对慢传输型便秘大鼠结肠平滑肌结构及Cajal间质细胞的影响
Effect of Electroacupuncture at "Tianshu"(ST 25) on Colonic Smooth Muscle Structure and Interstitial Cells of Cajal in Slow Transit Constipation Rats

孙建华;郭慧;陈璐;吴晓亮;李浩;裴丽霞;彭拥军;陆斌;
SUN Jian-hua,GUO Hui1,CHEN Lu2,WU Xiao-liang2,LI Hao2,PEI Li-xia,PENG Yong-jun,LU Bin(Department of Rehabilitation,Jiangsu Provincial Hospital of Chinese Medicine,Nanjing 210029,China;1Beijing Chaoyang District Anzhen Community Health Service Center,Beijing 100011;2Nanjing University of Chinese Medicine,Nanjing 210046)

摘要(Abstract):

目的:观察电针"天枢"穴对慢传输型便秘(STC)大鼠结肠平滑肌结构及Cajal间质细胞(ICC)的影响,探讨电针"天枢"穴治疗STC的作用机制。方法:56只Wistar大鼠,雌雄各半,随机选20只为正常组。饲喂复方苯乙哌啶饲料建立STC大鼠模型。造模成功的大鼠分为电针组和模型组,每组18只。电针组电针双侧"天枢"穴15 min,连续治疗14 d。采用HE染色法及免疫组化法观察各组大鼠结肠肠壁形态结构及结肠ICC的表达。结果:①HE染色中模型组大鼠肠黏膜的腺体萎缩,腺泡减少,分布稀疏,其平滑肌厚度与正常大鼠比较明显变薄(P<0.05);电针组大鼠结肠肠壁形态结构与正常组类似,其平滑肌厚度与模型组比较明显变厚(P<0.05)。②免疫组化染色中模型组大鼠ICC形态及分布与正常组类似,但着色较淡、模糊,网络结构不连续,其细胞计数与正常组比较明显减少(P<0.05),平均吸光度值与正常组比较明显降低(P<0.05);电针组结肠ICC数量接近正常组大鼠(P>0.05),与模型组比较明显升高(P<0.05)。结论:STC大鼠结肠功能障碍可能与结肠平滑肌结构和ICC病理改变有关。电针可使大鼠结肠肠壁平滑肌结构与ICC趋于正常,可能是其治疗STC的机制之一。
Objective To observe the effect of electroacupuncture(EA) of "Tianshu"(ST 25) on colonic smooth muscle structure and interstitial cells of Cajal(ICC) in slow transit constipation(STC) rats,so as to investigate its mechanism underlying improving STC.Methods Wistar rats(n=56) were randomly allocated to control(n=20),model and EA groups(n=18/group).STC model was established by feeding the rats with forage containing compound Diphenoxylate.EA(0.8-1.3 mA,2 Hz/15 Hz) was applied to bilateral "Tianshu"(ST 25) for 15 min,once daily for 14 days.The treatment was given beginning from the next day on after the rat's first black stool excretion was found.The colonic smooth muscle structure was observed under microscope after sectioning and HE staining.The immunoactivity(average optical density,OD) of intestinal immuno-reaction(IR) po-sitive ICC product was detected by immunohistochemistry.Results ①Microscopic observation displayed that after mode-ling,the rat's intestinal mucosa gland showed atrophy with reduction in the acinar number,being sparse in distribution;and the smooth muscle thickness was obviously thinner compared with the normal control group(P<0.05).Whereas this situation of the colonic smooth muscle in the EA group was close to that of the normal control group.Compared with the model group,the thickness of the intestinal smooth muscle in the EA group was significantly bigger(P<0.05).②Immunohistochemical results showed that the average OD values and number of IR-positive colonic ICC in the model group were obviously decreased in comparison with the normal control group(P<0.05).Compared with the model group,the average OD values and the number of IR-positive colonic ICC in the EA group were upregulated considerably(P<0.05).Conclusion EA of bilateral "Tianshu"(ST 25) is able to improve STC-induced structural changes of the colonic smooth muscle,and upregulate expression of colonic ICC in STC rats,which may be responsible for its efficacy in improving STC.

关键词(KeyWords): 电针;天枢;慢传输型便秘;结肠平滑肌结构;Cajal间质细胞
Electroacupuncture;Tianshu(ST 25);Slow transit constipation;Colonic smooth muscle structure;Interstitial cells of Cajal

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(No.30973794)

作者(Author): 孙建华;郭慧;陈璐;吴晓亮;李浩;裴丽霞;彭拥军;陆斌;
SUN Jian-hua,GUO Hui1,CHEN Lu2,WU Xiao-liang2,LI Hao2,PEI Li-xia,PENG Yong-jun,LU Bin(Department of Rehabilitation,Jiangsu Provincial Hospital of Chinese Medicine,Nanjing 210029,China;1Beijing Chaoyang District Anzhen Community Health Service Center,Beijing 100011;2Nanjing University of Chinese Medicine,Nanjing 210046)

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DOI: 10.13702/j.1000-0607.2011.03.003

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