针刺研究

2021, v.46(09) 721-727

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电针对脑缺血大鼠胼胝体髓鞘相关蛋白表达的影响
Effects of electroacupuncture on myelin-related protein in corpus callosum of rats with focal cerebral ischemia

董文青;缪化春;吴锋;李怀斌;
DONG Wen-qing;MIAO Hua-chun;WU Feng;LI Huai-bin;Department of Anatomy, Wannan Medical College;

摘要(Abstract):

目的:观察电针对局灶性脑缺血大鼠胼胝体髓鞘碱性蛋白(MBP)、髓鞘相关生长抑制因子(Nogo-A)及其受体(NgR)表达的影响,探讨电针对缺血性脑白质损伤的神经保护作用机制。方法:将40只雄性SD大鼠随机分为正常组、假手术组、模型组和电针组,每组10只。采用大脑中动脉栓塞法(MCAO)复制局灶性脑缺血大鼠模型。电针组电针"百会"、左侧"足三里",1次/d,每次30 min,连续14 d。采用神经功能评分和胶带去除实验评价神经功能缺损情况;苏木精-伊红染色观察胼胝体髓鞘的病理学改变;固蓝(LFB)染色观察胼胝体髓鞘的完整性;免疫组织化学染色法检测MBP、Nogo-A和NgR在胼胝体中的表达;Western blot法检测胼胝体中MBP、Nogo-A和NgR的相对表达量。结果:假手术组与正常组各项指标的差异无统计学意义(P>0.05)。与假手术组比较,模型组大鼠神经功能评分升高(P<0.05),胶带接触和去除时间明显延长(P<0.001);缺血侧胼胝体区纤维排列紊乱,有大量髓鞘丢失,囊泡面积明显增大(P<0.01),LFB染色强度降低(P<0.05);MBP表达下降(P<0.01),Nogo-A及其受体NgR的表达增多(P<0.01)。与模型组比较,电针组大鼠在脑缺血第3、7、14天时神经功能缺损评分降低(P<0.05),胶带接触时间在第3、7天明显缩短(P<0.001),去除时间在第7、14天明显缩短(P<0.001);胼胝体形态接近正常,髓鞘结构较完整,囊泡面积明显减小(P<0.05),LFB染色强度增加(P<0.05);MBP表达升高(P<0.01),Nogo-A及其受体NgR表达降低(P<0.01)。结论:电针可对脑缺血后胼胝体髓鞘发挥保护作用,其机制可能与抑制Nogo-A及其受体NgR表达有关。
Objective To observe the effect of electroacupuncture(EA)on the expression of myelin basic protein(MBP), axon growth inhibitor Nogo-A and Nogo receptor(NgR) in corpus callosum of rats with focal cerebral ischemia, so as to explore the mechanism of EA underlying improving ischemic white matter injury. Methods Fourty male SD rats were randomly divided into normal, sham operation, model and EA groups, with 10 rats in each group. The focal cerebral ischemia rat model was established by occlusion of the middle cerebral artery(MCAO). EA was applied to "Baihui"(GV20) and "Zusanli"(ST36) on the left side for 30 min, once daily for 14 days. Neurological function score and the adhensive removal test were used to evaluate neurological deficit severity; Hematoxylin-esion staining was used to observe the pathological changes in myelin of corpus callosum and luxol fast blue(LFB) staining was used to observe the myelin of corpus callosum. Immunohistochemical staining and Western blot were used to detect the expressions of MBP、Nogo-A and NgR in the ischemic corpus callosum.Results After MCAO, the neurological function score was significantly increased(P<0.05), the time required for contact with tape and tape removal was longer(P<0.001), the intensity of LFB staining and the expression of MBP decreased, while the veside area and the expression of Nogo-A and its receptor NgR increased(P<0.01, P<0.05) in the model group relevant to the normal and sham operation groups. The fiber arrangement of the corpus callosum on the ischemic side was disordered and a large amount of myelin sheath was lost in the model group. Following the treatment, the neurological deficit score of EA group was gradually decreased and significantly decreased on the 3~(rd), 7~(th) and 14~(th) day(P<0.05), and the time to remove the adhesive tape was shortened at the 7~(th) and 14~(th) day(P<0.001). The shape of the corpus callosum in the EA group was close to normal, and the myelin structure was relatively complete. The intensity of LFB staining and the expression of MBP was increased(P<0.05, P<0.01) while the expression of Nogo-A and its receptor NgR were decreased in the EA group relevant to the model group(P<0.01). Conclusion EA can play a protective role in myelin of the corpus callosum after cerebral ischemia, which may be related to down-regulating the expressions of Nogo-A and NgR.

关键词(KeyWords): 电针;脑缺血;胼胝体;髓鞘碱性蛋白;髓鞘相关生长抑制因子
Electroacupuncture;Cerebral ischemia;Corpus callosum;Myelin basic protein;Axon growth inhibitor

Abstract:

Keywords:

基金项目(Foundation): 皖南医学院大学生科研基金项目(No.WK2020S62);皖南医学院重点科研项目(No.WK2019Z05);皖南医学院学术带头人后备人选人才项目(No.校政[2019]81号);; 安徽省高校优秀青年骨干人才国内访问研修项目(No.gxgnfx2018016)

作者(Author): 董文青;缪化春;吴锋;李怀斌;
DONG Wen-qing;MIAO Hua-chun;WU Feng;LI Huai-bin;Department of Anatomy, Wannan Medical College;

Email:

DOI: 10.13702/j.1000-0607.20210187

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