针刺研究

2018, v.43(03) 140-145

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

电针联合天麻素对阿尔茨海默病大鼠海马CA 1区沉默信息调节因子2同源蛋白1和过氧化物酶体增殖物激活受体γ辅激活子1 ɑ表达的影响
Electroacupuncture plus Gastrodin Improves Learning-memory Ability Possibly by Up-regulating Expression of SIRT 1 and PGC-1 α in Hippocampal CA 1 Region of Alzheimer's Disease Rats

黄锐;吴锋;赵健;李怀斌;丁见;熊克仁;
HUANG Rui;WU Feng;ZHAO Jian;LI Huai-bin;DING Jian;XIONG Ke-ren;Department of Anatomy,Wannan Medical College;

摘要(Abstract):

目的:探讨电针联合天麻素治疗阿尔茨海默病(AD)的作用机制。方法:SD大鼠随机分为正常组、假手术组、模型组、电针组、天麻素组和针药联合组,每组10只。腹腔注射D-半乳糖联合双侧海马注射β淀粉样蛋白1-40制备AD大鼠模型。电针组和针药联合组给予"百会""大椎"、双侧"足三里"穴电针刺激,每次30min,1次/d,连续4周;天麻素组和针药联合组腹腔注射天麻素注射液,每日1次,连续4周。Morris水迷宫检测各组大鼠学习记忆能力;尼氏染色观察海马CA 1区神经元形态;免疫组织化学法检测各组大鼠海马CA 1区沉默信息调节因子2同源蛋白1(SIRT 1)和过氧化物酶体增殖物激活受体γ辅激活子(PGC-1ɑ)的表达。结果:Morris水迷宫结果显示,与正常组及假手术组比较,模型组大鼠逃避潜伏期延长(P<0.05),平台象限停留时间百分比、穿台次数降低(P<0.05);与模型组比较,电针与天麻素及联合使用均可降低AD大鼠的逃避潜伏期(P<0.05),提高平台象限停留时间百分比(P<0.05),增加穿台次数(P<0.05);针药联合组的效果优于电针组及天麻素组(P<0.05)。尼氏染色结果显示,与正常组及假手术组比较,模型组大鼠海马CA 1区神经元数量减少,排列紊乱;各治疗组CA 1区神经元数量较模型组明显增多,排列规则。与正常组及假手术组比较,模型组大鼠海马CA 1区SIRT 1和PGC-1ɑ阳性表达水平降低(P<0.05);与模型组比较,电针组和天麻素组CA 1区SIRT 1和PGC-1ɑ阳性表达水平升高(P<0.05);针药联合组的表达水平高于电针组和天麻素组(P<0.05)。结论:电针与天麻素均能够改善AD大鼠的学习记忆能力,且电针联合天麻素的效果更为显著,提示其可能通过上调海马SIRT 1和PGC-1ɑ蛋白的表达,发挥对AD大鼠神经元的保护作用。
Objective To observe the effect of electroacupuncture(EA)combined with Gastrodin on learning-memory ability and expression of silent information regulator 2 homologous protein 1(SIRT 1)and peroxisome proliferator activated receptorγcoactivator(PGC-1α)of hippocampal CA 1 region in Alzheimer's disease(AD)rats,so as to explore its mechanism underlying improvement of AD.Methods Sixty male SD rats were randomly divided into normal control(normal),sham operation(sham),model,EA,Gastrodin and EA+ Gastrodin groups(n=10 in each).The AD model was established by intraperitoneal injection of D-Galactose(120 mg·kg~(-1)·d~(-1))combined with bilateral hippocampal injection ofβamyloid 1-40(Aβ1-40).EA was applied at"Baihui"(GV 20),"Dazhui"(GV 14)and"Zusanli"(ST 36)for 30 min,once daily for 4 weeks.For rats of the Gastrodin group and EA+ Gastrodin group,intraperitoneal injection of gastrodin(10 mg/kg)was conducted once daily for 4 weeks.Morris water maze tests were used to assess the rat's learning-memory ability.Nissl staining was used to assess the morphological changes of neurons in the hippocampal CA 1 area.The expression of SIRT 1 and PGC-1αof hippocampal CA 1 region was measured by immunohistochemical staining.Results 1)Morris water maze tests showed that,compared with the normal and sham group,the escape latency was significantly prolonged(P<0.05),and the percentage of platform quadrant residence duration and the platform crossing times were considerably decreased in the model group(P<0.05).After the intervention,the escape latency was obviously shortened(P<0.05),and the percentage of platform quadrant residence duration and the platform crossing times were markedly increased in the EA,Gastrodin and EA+Gastrodin groups relevant to the model group(P<0.05).2)Nissl staining showed that,in comparison with the normal group or sham group,the number of cells in the hippocampal CA 1 area was decreased and the arrangement was disorganized in the model group.The number of cells in CA 1 area was relatively higher in the3 treatment groups than in the model group.3)The expression levels of SIRT 1 and PGC-1αproteins in the hippocampal CA 1 area were significantly down-regulated in the model group than in the normal and sham groups(P<0.05).After the intervention,the expression levels of SIRT 1 and PGC-1αin the EA,Gastrodin and EA+Gastrodin groups were significantly up-regulated compared with the model group(P<0.05).The effects of EA+Gastrodin were significantly superior to those of simple EA and simple Gastrodin in shortening the escape latency,up-regulating the expression levels of SIRT 1 and PGC-1αas well as in increasing the percentage of platform quadrant residence time and platform crossing times(P<0.05).Conclusion Both EA and Gastrodin can improve the learning-memory ability of AD rats,which may be related to their effects in up-regulating the expression of SIRT 1 and PGC-1αand reducing neuronal injury in the CA 1 region of hippocampus,suggesting aprotective role of EA on hippocampal neurons.The effect of EA combined with Gastrodin is markedly better than that of EA and Gastrodin alone.

关键词(KeyWords): 电针;天麻素;阿尔茨海默病;沉默信息调节因子2同源蛋白1;过氧化物酶体增殖物激活受体γ辅激活子;海马CA 1区;学习记忆能力
Electroacupuncture;Gastrodin;Alzheimer's disease;Silent information regulator 2 homologous protein 1;Peroxisome proliferator activated receptor γ coactivator;Hippocampal CA 1;Learning-memory ability

Abstract:

Keywords:

基金项目(Foundation): 安徽省自然科学基金项目(No.1608085QH 223);; 安徽省熊克仁名师工作室(No.2014msgzs 152);; 安徽省高等学校自然科学研究重点项目(No.KJ 2015A227)

作者(Author): 黄锐;吴锋;赵健;李怀斌;丁见;熊克仁;
HUANG Rui;WU Feng;ZHAO Jian;LI Huai-bin;DING Jian;XIONG Ke-ren;Department of Anatomy,Wannan Medical College;

Email:

DOI: 10.13702/j.1000-0607.170635

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享