谭思由;黄俊;苏颖颖;陈文雁;孔高茵;刘景诗;魏来;

• 1:湖南师范大学附属第一医院/湖南省人民医院麻醉科
• 2:湖南省围术期加速康复麻醉临床医学研究中心

摘要(Abstract)：

目的:研究电针"肝俞""阳陵泉"对大鼠肝脏缺血再灌注损伤(HIRI)的保护作用和对肝脏组织高迁移率族蛋白1(HMGB1)表达的影响。方法:雄性SD大鼠随机分为假手术组、模型组、肝俞-阳陵泉组和非经非穴组,每组10只。采用无创动脉夹阻断供应肝中叶和左叶的动脉、静脉和胆管1 h,解除阻断后再灌注4 h以构建70%HIRI模型。肝俞-阳陵泉组取两侧"肝俞"和"阳陵泉",非经非穴组取两侧"肝俞"穴区旁开6～8 mm的非经非穴区肌肉结缔组织,此二组均在造模前给予电针刺激30 min。以HE染色法观察肝脏组织损伤情况,全自动生化分析仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平,酶联免疫法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和HMGB1水平,免疫组织化学法和蛋白质免疫印迹法检测肝脏组织HMGB1蛋白表达,荧光定量PCR检测肝脏组织HMGB1 mRNA表达。结果:与假手术组比较,造模后大鼠肝脏损伤评分及血清ALT、AST、TNF-α、IL-6和HMGB1含量均显著升高(P<0.01),肝脏组织中的HMGB1阳性细胞数、HMGB1 mRNA及蛋白表达均明显升高(P<0.01)。与模型组比较,肝俞-阳陵泉组大鼠肝脏损伤评分及血清ALT、AST、TNF-α、IL-6和HMGB1含量均降低(P<0.05),肝脏组织中的HMGB1阳性细胞数、HMGB1 mRNA及蛋白表达降低(P<0.05)。非经非穴组大鼠肝脏损伤评分及血清ALT、AST、TNF-α、IL-6和HMGB1含量均高于肝俞-阳陵泉组(P<0.05),肝脏组织中HMGB1阳性细胞数、HMGB1 mRNA及蛋白表达均高于肝俞-阳陵泉组(P<0.05)。结论:电针"肝俞""阳陵泉"可减轻大鼠肝脏缺血再灌注损伤,这种保护作用可能是通过抑制HMGB1从胞核向胞质移位和释放、下调相关炎性因子表达来发挥的。

关键词(KeyWords)： 电针;肝脏缺血再灌注损伤;高迁移率族蛋白1;炎性因子

Abstract:

Keywords:

基金项目(Foundation): 湖南省教育厅项目(No.16C0971);; 湖南省中医药管理局项目(No.202071)

作者(Author): 谭思由;黄俊;苏颖颖;陈文雁;孔高茵;刘景诗;魏来;

Email:

DOI: 10.13702/j.1000-0607.200374

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