针刺研究

2014, v.39(01) 58-62+82

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麦粒灸对阿尔茨海默病小鼠学习记忆能力及脑内淀粉样蛋白沉积的影响
Effects of Grain-sized Moxibustion on Learning and Memory Ability and Amyloid Deposition of Transgenic Alzheimer's Disease Mice

余静;楚佳梅;高灵爱;张永生;包烨华;
YU Jing;CHU Jia-mei;GAO Ling-ai;ZHANG Yong-sheng;BAO Ye-hua;Zhejiang University of Chinese Medicine;Department of Acu-moxibustion,Hangzhou Traditional Chinese Medicine Hospital;Yuhang District Fifth People's Hospital of Hangzhou;

摘要(Abstract):

目的:观察麦粒灸对阿尔茨海默病(AD)小鼠学习记忆能力及脑内淀粉样蛋白(Aβ)沉积的影响,探讨麦粒灸防治AD的机制。方法:选取APP/PS 1双转基因AD小鼠17只,随机分为模型组9只和治疗组8只,同龄、同背景的C 57BL/6J阴性纯合子小鼠9只为正常组。治疗组取双侧"心俞""肾俞"行麦粒灸治疗,每日1次,10次为1个疗程,共治疗9个疗程。观察逃避潜伏期、穿越平台的次数及目标象限停留时间百分比来评价小鼠的学习记忆能力,刚果红染色法观察小鼠脑内Aβ沉积的变化。结果:与正常组比较,模型组逃避潜伏期延长,穿越平台次数减少,目标象限停留时间百分比减少(P<0.05);模型组小鼠大脑额叶皮层和海马区呈现大小不一的Aβ沉积物。治疗后,治疗组小鼠学习记忆能力提高,与模型组小鼠比较,逃避潜伏期缩短,穿越平台次数增多,目标象限停留时间百分比增加(P<0.05);治疗组小鼠大脑额叶皮层区和海马区Aβ沉积较模型组减少(P<0.05)。结论:麦粒灸疗法可显著改善APP/PS 1转基因AD小鼠的学习记忆能力,其作用机制可能与有效抑制大脑额叶皮层区和海马区Aβ沉积有关。
Objective To observe the effect of grain-sized moxibustion at"Xinshu"(BL 15)and"Shenshu"(BL 23)on memory-learning ability and amyloid deposition in transgenic Alzheimer's disease(AD)mice.Methods Seventeen amyloid precursor protein(APP)/presenilin(PS)1(APP+/PS 1+)double transgenic 6799mice aged 3-4weeks were randomly divided into model group(n=9)and moxibustion group(n=8).Nine wide-type(C 57BL/6J)female mice were used as the normal control group.Moxibustion(ignited grain-sized moxa cone)was applied to bilateral"Xinshu"(BL 15)and"Shenshu"(BL 23)for about 30s,once a day for 9courses(10days constitute a therapeutic course,with 2days' break between every two courses).Morris water maze tests were performed to detect the mice's learning-memory ability.The alterations of beta-amyloid deposition(number of the positive plaques)in the cerebral cortex and hippocampus were detected by using an imaging analysis system following Congo red staining of the cerebral tissue sections.Results Compared with the normal group,the average escape latency of place navigation tests was significantly increased(P<0.05),and the target-platform crossing times and percentage of dwell time in the target quadrant of the spatial exploring tests were notably decreased in the model group(P<0.05).After moxibustion intervention,the escape latency was remarkably shortened,and the target-platform crossing times and dwell time percentage were obviously increased in the moxibustion group in comparison with the model group(P<0.05),suggesting an improvement of the learning-memory ability after moxibustion.Results of Congo red staining of the cerebral tissue showed that there were many irregular,uneven staining positive plaques in the cerebral cortex and hippocampus of AD mice in the model group.Compared with the model group,the positive plaque numbers in both cerebral cortex and hippocampus were considerably reduced in the moxibustion group(P<0.05).Conclusion Grain-sized moxibustion at"Xinshu"(BL 15)and"Shenshu"(BL 23)can ameliorate learning-memory ability and restrain the formation of amyloid deposition in AD mice.

关键词(KeyWords): 麦粒灸;APP/PS1转基因鼠;淀粉样蛋白沉积;学习记忆能力;阿尔茨海默病
Grain-sized moxibustion;APP+/PS 1+transgenic mice;Amyloid deposition;Learning-memory ability;Alzheimer's disease

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金(No.LY 13H270002);; 杭州市科技发展计划项目(No.2011KJJ 16)

作者(Author): 余静;楚佳梅;高灵爱;张永生;包烨华;
YU Jing;CHU Jia-mei;GAO Ling-ai;ZHANG Yong-sheng;BAO Ye-hua;Zhejiang University of Chinese Medicine;Department of Acu-moxibustion,Hangzhou Traditional Chinese Medicine Hospital;Yuhang District Fifth People's Hospital of Hangzhou;

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DOI: 10.13702/j.1000-0607.2014.01.011

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