针刺研究

2017, v.42(03) 202-208+216

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基于代谢组学研究热补针法治疗类风湿关节炎寒证家兔的作用机制
Mechanism of Heat-reinforcing Needling for Rheumatoid Arthritis Rabbits with Cold Syndrome Based on Metabonomics

杜小正;袁博;张星华;田亮;张婷卓;李兴兰;张枫帆;王金海;
DU Xiao-zheng;YUAN Bo;ZHANG Xing-hua;TIAN Liang;ZHANG Ting-zhuo;LI Xing-lan;ZHANG Feng-fan;WANG Jin-hai;School of Acu-moxibustion and Massage,Gansu University of Chinese Medicine;Department of Acu-moxibustion,Gansu Provincial Hospital of Traditional Chinese Medicine;Department of Chinese Medicine,the Second Hospital of Lanzhou University;

摘要(Abstract):

目的:采用代谢组学技术探讨热补针法治疗类风湿关节炎(RA)寒证的特异性作用机制。方法:青紫蓝家兔随机分为正常组、模型组、平补平泻组、捻转补法组、热补针法组,每组8只。以卵蛋白诱导联合低温冷冻法建立RA寒证模型。各治疗组分别运用平补平泻法、捻转补法、热补针法于双侧"足三里"穴进行针刺干预,治疗30min,每日1次,共治疗7d。治疗后观察各组家兔的痛阈值以及关节表面温度,采用气相色谱-质谱(GC-MS)检测结合模式识别法分析家兔血清中内源性代谢物的变化。结果:模型组家兔痛阈、关节表面温度较正常组降低(P<0.05),各针刺组干预后痛阈、关节表面温度较模型组升高(P<0.05),热补针法组高于平补平泻组、捻转补法组(P<0.05)。与正常组相比,模型组家兔血液代谢物中亮氨酸、缬氨酸、异亮氨酸、花生四烯酸的含量升高(P<0.05),α-酮戊二酸、柠檬酸、琥珀酸、葡萄糖、纤维糖、棕榈酸、硬脂酸、乳糖、d-核糖、D-甘露糖的含量降低(P<0.05)。与模型组比较,各针刺组血清中代谢物均出现不同程度的回调(P<0.05),且热补针法组对α-酮戊二酸、柠檬酸、琥珀酸、葡萄糖、纤维糖、d-核糖、D-甘露糖的回调明显优于平补平泻组和捻转补法组(P<0.05)。结论:热补针法治疗RA具有确切的疗效,可能与对三羧酸循环与糖代谢的相对特异性调控有关。
Objective To explore the specific mechanism of heat-reinforcing needling(HRN)for rheumatoid arthritis(RA)rabbits with cold syndrome based on metabolomics.Methods A total of 40 healthy rabbits were randomly divided into a normal group,a model group,a reinforcing-reducing needling(RRN)group,a twirling-reinforcing needling(TRN)group and a HRN group(n=8rabbits/group).The RA model with cold syndrome was established with ovalbumin and freezing.Except the normal and model groups,RRN,TRN and HRN were given at"Zusanli"(ST 36)in the corresponding acupuncture groups for 30 min,once a day for 7days.After the interventions,the pain threshold and the local skin temperature of each group were observed.The endogenous metabolites in the serum were analyzed by the gas chromatography mass spectrometry(GC-MS)technology combined with the pattern-recognition method.Results The pain threshold and the local skin temperature in the model group were lower than those in the normal group(P<0.05).The pain thresholds and the local skin temperatures in the three acupuncture groups were higher than those in the model group after interventions(P<0.05),and those in the HRN group were obviously higher than those in the RRN and TRN groups(P<0.05).The serum metabolites of leucine,valine,isoleucine,and arachidonic acid in the model group were increased compared with those in the normal group(P<0.05),and the metabolites ofα-ketoglutaric acid,citric acid,succinic acid,glucose,inositol,palmitic acid,stearic acid,lactose,d-ribose,and D-mannose were decreased(P<0.05).The above-mentioned metabolites in the three acupuncture groups were significantly reversed relevant to the model group(P<0.05),and the effect of HRN group was significantly superior to those of the RRN and TRN groups in regulatingα-ketoglutaric acid,citric acid,succinic acid,glucose,inositol,d-ribose,and D-mannose(P<0.05).Conclusion HRN for RA with cold syndrome is effective,which may be related to the specific regulation for the krebs cycle and glycometabolism.

关键词(KeyWords): 热补针法;类风湿关节炎;代谢组学
Heat-reinforcing needling;Rheumatoid arthritis;Metabonomics

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.81260558);; 国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(No.2305135901)

作者(Authors): 杜小正;袁博;张星华;田亮;张婷卓;李兴兰;张枫帆;王金海;
DU Xiao-zheng;YUAN Bo;ZHANG Xing-hua;TIAN Liang;ZHANG Ting-zhuo;LI Xing-lan;ZHANG Feng-fan;WANG Jin-hai;School of Acu-moxibustion and Massage,Gansu University of Chinese Medicine;Department of Acu-moxibustion,Gansu Provincial Hospital of Traditional Chinese Medicine;Department of Chinese Medicine,the Second Hospital of Lanzhou University;

DOI: 10.13702/j.1000-0607.2017.03.003

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