艾灸治疗类风湿性关节炎抗炎免疫作用机理的研究Study on the Action Mechanism of Moxibustion in Anti-inflammati on and Immunoregulation in Rheumatoid Arthritis Rats
唐照亮,宋小鸽,章复清,王宁新,徐斌,侯正明,陈全珠,袁静,陈向涛,张道芹,王元勋
TANG Zhao-liang, SONG Xiao-ge, ZHANG Fu-qing, WANG Ning-x in 1, XU Bin 2, HOU Zheng-ming, CHEN Quan-zhu, YUAN Jing, CHEN Xiang-tao, ZHANG Dao -qin, WANG Yuan-xun (Institute of Acupuncture and Moxibustion, Anhui College of T CM, Hefei, 230038; 1The Affiliated Hospital of Anhui College of TCM,Hefei,230031; 2Nanjing University of TCM,Jiangsu Province, 210029)
摘要(Abstract):
为了探讨艾灸治疗RA抗炎免疫作用的机理 ,我们建立免疫性炎症———大鼠佐剂性关节炎模型等 ,从神经 内分泌 免疫调控入手 ,在整体、器官、细胞水平开展灸治RA的实验研究。结果艾灸“肾俞”、“足三里”穴具有抗炎免疫作用 ,其机理与灸疗抑制TNF、IL 1等炎症因子的释放 ,增强与改善机体的免疫功能 ,保护胸腺、脾脏等免疫器官 ,纠正炎症时自由基代谢的紊乱 ,调整NO、NE、5 HT神经递质的失衡 ,促进内环境稳定等多方面因素有关。实验证实 ,外周交感神经参与艾灸对免疫的调节 ;灸疗的部分作用是通过肾上腺皮质系统发挥的 ;海马可能是灸疗信息中枢整合的重要环节 ;HPA轴是灸疗作用中一条重要的神经体液性途径 ;松果体 (MT)可能是艾灸抗炎免疫的一个高位调节点。提示灸疗抗炎免疫作用是其治疗RA的主要机制之一
In the present paper, the authors summarize their resu l ts of study on the mechanism of moxibustion of "Shenshu"(BL 23) and "Zusanli"( ST 36) for resisting inflammation and modulating immune function in the treatment of rheumatoid arthritis (AA) rats.1)Anti-inflammatory effect: In AA rats, afte r 3 sessions of moxibustion, the c ircumferences of the swollen ankle of BL-23 group (2.33±0.19 cm) and ST-3 6 grou p (2.35±0.11 cm) were smaller than that of control group (2.69±0.22 cm) . In de layed general polyarthritis rats of these two moxibustion groups, the number o f inflammatory nodes at the ear and tail was less than that of control group. 2) Reducing release of inflammatory mediators: Following moxibustion, the elevated contents of blood tumor necrosis factor (TNF) and IL-1 in AA rats decreased sig n ificantly compared with control group ( P <0.05). Moxibustion could also reduc e th e release of histamine and 5-HT. 3) Modulating free radical metabolism: In AA r a ts, moxibustion could reduce blood nitric oxide (NO) and malondiadehyde levels a nd raise SOD activity. 4) Regulating immunofunction: Moxibustion could cause th e endogenous IL-2 level thymus index to raise and reduce the secretion of IL-1 o f the over-excited megoxycytes and lower the spleen index in AA rats. Following removal of the adrenal gland or sympathetectomy (with 6-OHDA), the effects of m o xibustion in modulating immunofunction were weakened significantly. 5) Regulatin g central neurotransmitter level: In AA rats, following moxibustion, the elevate d NE and 5-HT contents in the hypothalamus were upregulated, while the elevated NO level was downregulated. 6) Involvement of hippocampus and pineal gland in th e effects of moxibustion: In AA rats, after injection of 6-OHDA for destruction of adrenagic nerve, the effect of moxibustion on reduction of inflammatory respo nse of the ankle was weakened. In addition, the decreased level of melatonin (MT ) of the pineal gland tissue in AA rats was raised by moxibustion. In a word, mo xibustion induced anti-inflammation and modulation of immunofunction may contri bute to its therapeutic effect in improving rheumatoid arthritis.
关键词(KeyWords):
艾灸;类风湿性关节炎;佐剂性关节炎;抗炎;免疫调节
Experimental arthritis Moxibustion Anti-inflammati on Immunoregulation
基金项目(Foundation): 安徽省自然科学基金;; 江苏省针灸学重点实验室开放课题;; 国家自然科学基金 (No 3 0 171183 )资助项目
作者(Author):
唐照亮,宋小鸽,章复清,王宁新,徐斌,侯正明,陈全珠,袁静,陈向涛,张道芹,王元勋
TANG Zhao-liang, SONG Xiao-ge, ZHANG Fu-qing, WANG Ning-x in 1, XU Bin 2, HOU Zheng-ming, CHEN Quan-zhu, YUAN Jing, CHEN Xiang-tao, ZHANG Dao -qin, WANG Yuan-xun (Institute of Acupuncture and Moxibustion, Anhui College of T CM, Hefei, 230038; 1The Affiliated Hospital of Anhui College of TCM,Hefei,230031; 2Nanjing University of TCM,Jiangsu Province, 210029)
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DOI:
参考文献(References):
- 1 翁文水针灸治疗类风湿性关节炎近况中国针灸,1997,17(8):507
- 2 孙 华针灸治疗类风湿性关节炎研究进展中国针灸,2001,21(3):186
- 3 袁 静,唐照亮,宋小鸽,等艾灸对大鼠佐剂性关节炎影响的形态学观察针刺研究,1997,22(3):179
- 4 唐照亮,宋小鸽,李 俊,等艾灸抗炎免疫作用的实验观察与分析针刺研究,1996,21(2):67
- 5 唐照亮,宋小鸽,侯正明,等灸疗抗炎免疫作用的实验研究中国针灸,1997,17(4):233
- 6 唐照亮,宋小鸽,章复清,等艾灸关节炎大鼠抗炎消肿作用及对细胞因子、自由基的影响安徽中医学院学报,2001,20(5):34
- 7 唐照亮,宋小鸽,侯正明,等艾灸抗炎免疫作用的基础实验研究针刺研究,1997,22(3):168
- 8 唐照亮,宋小鸽,侯正明,等艾灸抗炎作用对炎症介质影响的实验研究针刺研究,1999,24(3):212
- 9 唐照亮,章复清,宋小鸽,等艾灸对实验性炎症大鼠血清SOD活性和MDA含量的影响安徽中医学院学报,1998,17(5):44
- 10 宋小鸽,唐照亮,侯正明,等艾灸对实验性炎症大鼠抗炎作用和红细胞免疫功能的影响中国中医药信息杂志,1999,6(12):31
- 11 SongXiaoge,TangZhaoliang,HouZhengming,etalEffectofmoxibustionontheimmunityandthecentralnervoustransmittersinmicewithyangdeficency.WorldJAcupMox,1999,9(4):47
- 12 唐照亮,陈淼和,宋小鸽,等艾灸抗小鼠S180实体瘤的实验研究针刺研究,1999,24(1):60
- 13 唐照亮,宋小鸽,侯正明,等艾灸肾俞延缓衰老的实验研究安徽中医学院学报,1999,18(5):53
- 14 唐照亮,宋小鸽,侯正明,等大鼠肾上腺摘除对艾灸抗炎免疫作用的影响中国中医药信息杂志,2000,7(9):29
- 15 唐照亮,余新欣,陈全珠,等6羟多巴胺化学损毁小鼠外周交感神经对艾灸免疫调节作用的影响针刺研究,001,26(4):299
- 16 唐照亮,宋小鸽,陈全珠,等艾灸抗炎免疫作用及对神经递质影响的实验研究中国中医基础医学杂志,2000,6(9):53
- 17 唐照亮,宋小鸽,侯正明,等大鼠海马内微量注射6羟多巴胺对艾灸抗炎免疫作用的影响针刺研究,2000,21(7):184
- 18 唐照亮,宋小鸽,李 俊,等艾灸抗炎免疫作用中松果腺褪黑素高位调节的研究中国针灸,2001,21(7):429
- 唐照亮
- 宋小鸽
- 章复清
- 王宁新
- 徐斌
- 侯正明
- 陈全珠
- 袁静
- 陈向涛
- 张道芹
- 王元勋
TANG Zhao-liang - SONG Xiao-ge
- ZHANG Fu-qing
- WANG Ning-x in 1
- XU Bin 2
- HOU Zheng-ming
- CHEN Quan-zhu
- YUAN Jing
- CHEN Xiang-tao
- ZHANG Dao -qin
- WANG Yuan-xun (Institute of Acupuncture and Moxibustion
- Anhui College of T CM
- Hefei
- 230038
- 1The Affiliated Hospital of Anhui College of TCM
- Hefei
- 230031
- 2Nanjing University of TCM
- Jiangsu Province
- 210029)
- 唐照亮
- 宋小鸽
- 章复清
- 王宁新
- 徐斌
- 侯正明
- 陈全珠
- 袁静
- 陈向涛
- 张道芹
- 王元勋
TANG Zhao-liang - SONG Xiao-ge
- ZHANG Fu-qing
- WANG Ning-x in 1
- XU Bin 2
- HOU Zheng-ming
- CHEN Quan-zhu
- YUAN Jing
- CHEN Xiang-tao
- ZHANG Dao -qin
- WANG Yuan-xun (Institute of Acupuncture and Moxibustion
- Anhui College of T CM
- Hefei
- 230038
- 1The Affiliated Hospital of Anhui College of TCM
- Hefei
- 230031
- 2Nanjing University of TCM
- Jiangsu Province
- 210029)