针刺研究

2011, v.36(01) 7-11

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“嗅三针”对阿尔茨海默病大鼠海马Bcl-2和Bax表达的干预效应
Effect of "Xiusanzhen" on Expression of Hippocampal Bcl-2 and Bax Proteins in Alzheimer Disease Rats

刘智斌;牛文民;杨晓航;牛晓梅;王渊;
LIU Zhi-bin,NIU Wen-min,YANG Xiao-hang1,NIU Xiao-mei1,WANG Yuan1(Department of Acu-moxibustion and Tuina,Shaanxi College of Chinese Medicine,Xianyang 712046,China;1The Affiliated Hospital of Shaanxi College of Chinese Medicine,Xianyang 712020)

摘要(Abstract):

目的:探讨"嗅三针"通过嗅觉通路对阿尔茨海默病(AD)模型大鼠海马神经元细胞凋亡的影响,以阐明其治疗AD的作用机制。方法:成年SD雄性大鼠40只,随机分为正常对照组、AD模型组、嗅神经切断嗅三针组、嗅三针组,每组10只。β-淀粉样蛋白(Aβ)1-40肽段注射法制作AD大鼠模型,嗅神经切断术造大鼠嗅觉通路阻断模型。"印堂"穴及两侧"迎香"穴交替电针刺激行"嗅三针"治疗,每次10 min,每周治疗5次,共治疗6周。免疫组化法检测海马Bcl-2和Bax表达。结果:AD模型组较正常对照组Bcl-2阳性细胞减少(P<0.05),Bax阳性细胞表达增多(P<0.01);嗅三针组较AD模型组Bcl-2阳性细胞明显增多(P<0.01),Bax阳性细胞显著减少(P<0.01);嗅神经切断嗅三针组与AD模型组比较Bcl-2、Bax阳性细胞变化不明显(P>0.05)。结论:"嗅三针"能够调节海马Bcl-2和Bax的表达,其治疗效应的发挥依赖于嗅觉传导通路的完整性。
Objective To observe the effect of electroacupuncture(EA) of "Xiusanzhen"[3 acupoints,i.e.,bilateral "Yingxiang"(LI 20) and "Yintang"(EX-HN 3)] on expression of B-cell lymphoma/leukemia-2(Bcl-2,an antiapoptosis protein) and Bax(a protein for promoting apoptosis) in the hippocampus in Alzheimer disease(AD)rats,so as to explore its clinical mecha-nisms underlying relieving AD.Methods A total of 40 Sprague Dawley rats were randomly divided into normal control,AD mo-del,EA and olfactory nerve transaction(ONT)+EA groups,with 10 rats in each.AD model was established by injection of β-amyloid(Aβ)1-40(2 μL containing 10 μg) into the hippocampus(Bregma: 3.5 mm,L: 2.0 mm,H:-2.8 mm below dura),and the ON was sectioned by using a fine needle.EA(80-100 Hz,1-3 mA) was applied to bilateral LI 20 and EX-HN 3 for 10 min,once daily(except Saturdays and Sundays) for 6 weeks.The expression of Bcl-2 and Bax of hippocampus was measured by immunohistochemistory.Results Compared with the normal control group,the expression of Bcl-2 of hippocampus was significantly lower in the AD model group(P<0.05),and that of Bax in AD model group was much higher(P<0.01).In comparison with the model group,the expression of hippocampal Bcl-2 was up-regulated significantly(P<0.01),and that of hippocampal Bax protein down-regulated considerably in the EA group(P<0.01).No significant differences were found between AD model and ONT+EA groups in the expression of hippocampal Bcl-2 and Bax proteins(P>0.05),suggesting a necessary premise condition for EA in regulating hippocampal Bcl-2 and Bax expression.Conclusion "Xiusanzhen" can regulate the expression of hippocampal Bcl-2 and Bax proteins in AD rats,which may contribute to its clinical effect in relieving AD,and the therapeutic effect depends on the integrity of the olfactory nerve pathway.

关键词(KeyWords): 阿尔茨海默病;电针;嗅三针;海马;嗅神经通路;Bcl-2;Bax
Alzheimer disease;Electroacupuncture;Xiusanzhen;Hippocampus;Olfactory nerve pathway;Bcl-2;Bax

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(No.30973792)

作者(Author): 刘智斌;牛文民;杨晓航;牛晓梅;王渊;
LIU Zhi-bin,NIU Wen-min,YANG Xiao-hang1,NIU Xiao-mei1,WANG Yuan1(Department of Acu-moxibustion and Tuina,Shaanxi College of Chinese Medicine,Xianyang 712046,China;1The Affiliated Hospital of Shaanxi College of Chinese Medicine,Xianyang 712020)

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DOI: 10.13702/j.1000-0607.2011.01.003

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