宋越;马良宵;甘莹莹;王俊翔;母杰丹;郭孟玮;任晓暄;于文颜;田园;钱旭;孙天祎;

• 1:北京中医药大学针灸推拿学院

摘要(Abstract)：

目的:观察直刺、沿皮刺"三阴交"穴对寒凝证类痛经大鼠血清、子宫、脊髓及下丘脑中精氨酸加压素(AVP)及其受体(AVPR1A、AVPR1B)的影响,探讨不同刺法即刻镇痛效应的机制。方法:雌性SD大鼠随机分为空白组、模型组、直刺组、沿皮刺组,每组10只。采用全身冷冻加雌二醇注射法制备寒凝证类痛经模型。直刺组、沿皮刺组分别直刺或沿皮刺"三阴交"穴,留针20 min。记录各组大鼠的扭体反应情况。采用ELISA法、Western blot法、荧光定量PCR法分别检测大鼠血清、子宫、脊髓以及下丘脑的AVP含量、AVPR1A与AVPR1B蛋白及mRNA表达。结果:与空白组比较,模型组扭体潜伏期缩短(P<0.05),20 min内扭体评分显著升高(P<0.01);与模型组比较,直刺及沿皮刺组扭体潜伏期均明显延长(P<0.01),20 min内扭体评分显著降低(P<0.01)。与空白组比较,模型组血清和子宫AVP含量升高(P<0.05,P<0.01)、脊髓和下丘脑AVP含量降低(P<0.01,P<0.05),子宫AVPR1A蛋白及mRNA表达升高(P<0.01,P<0.05)、脊髓AVPR1A蛋白及mRNA降低(P<0.05,P<0.01),下丘脑AVPR1B蛋白及mRNA表达降低(P<0.01)。与模型组比较,直刺组血清AVP含量降低(P<0.05),下丘脑AVPR1B蛋白表达升高(P<0.05);直刺与沿皮刺组子宫AVP含量均显著降低(P<0.01),下丘脑AVP含量均升高(P<0.05),子宫AVPR1A蛋白及其mRNA表达均降低(P<0.01,P<0.05)。直刺组与沿皮刺组各指标间差异无统计学意义(P>0.05)。结论:直刺、沿皮刺"三阴交"穴对类痛经大鼠均显示出即刻镇痛效应,其机制可能与针刺对子宫及下丘脑AVP及其受体的调节有关。

关键词(KeyWords)： 直刺;沿皮刺;精氨酸加压素;精氨酸加压素受体;痛经

Abstract:

Keywords:

基金项目(Foundation): 北京中医药大学科研纵向发展基金项目(No.2018-ZXFZJJ-010);; 国家自然科学基金项目(No.81774417)

作者(Author): 宋越;马良宵;甘莹莹;王俊翔;母杰丹;郭孟玮;任晓暄;于文颜;田园;钱旭;孙天祎;

Email:

DOI: 10.13702/j.1000-0607.200184

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