王金海;张婷卓;赵敏;鲍英存;鄢琦;张振昶;杜小正;姜华;田亮;袁博;李兴兰;
WANG Jin-hai;ZHANG Ting-zhuo;ZHAO Min;BAO Ying-cun;YAN Qi;ZHANG Zhen-chang;DU Xiao-zheng;JIANG Hua;TIAN Liang;YUAN Bo;LI Xing-lan;Department of Traditional Chinese Medicine,Second Hospital of Lanzhou University;School of Acupuncture-moxibustion and Massage,Gansu University of Traditional Chinese Medicine;Department of Neurology,Second Hospital of Lanzhou University;

• 1:兰州大学第二医院中医科
• 2:甘肃中医药大学针灸推拿学院
• 3:兰州大学第二医院神经内科

摘要(Abstract)：

目的:观察头穴针刺对局灶性脑缺血大鼠海马旁回核因子(NF)-κB p 65mRNA、IκB mRNA及白介素(IL)-1β、肿瘤坏死因子(TNF)-α表达的影响,探讨头穴针刺调控缺血性脑血管病炎性反应的机制。方法:SD大鼠随机分为正常组、模型组、药物组及头针组,每组16只。采用线栓法建立大鼠急性局灶性脑缺血模型。药物组腹腔注射吡咯烷二硫代氨基甲酸铵盐(100mg·kg^(-1)·d(-1)·d^(-1)),1次/d,共7d。头针组取双侧顶颞前斜线和顶颞后斜线行快速捻转透刺治疗,每次30min,1次/d,共7d。干预前后进行神经功能缺损评分及神经行为学评分。RT-PCR法检测海马旁回NF-κB p 65mRNA、IκB mRNA的表达,免疫组织化学法检测海马旁回IL-1β、TNF-α的表达。结果:与正常组比较,模型组大鼠神经功能缺损评分和神经行为学评分明显增高(P<0.01),海马旁回NF-κB p 65 mRNA、IL-1β、TNF-α的表达均明显增高(P<0.01),IκB mRNA表达显著降低(P<0.01)。与模型组比较,头针组和药物组大鼠神经功能缺损评分和神经行为学评分明显降低(P<0.01),海马旁回NF-κB p 65 mRNA、IL-1β、TNF-α表达均明显降低(P<0.05),IκB mRNA表达明显增高(P<0.05)。结论:上调缺血后脑组织IκB的表达,抑制NF-κB的解离,进而降低炎性因子IL-1β、TNF-α的表达是头穴针刺控制缺血性脑血管病炎性反应的分子机制之一。
Objective To observe the effect of scalp-acupuncture intervention on the expression of parahippocampal factor-κB p 65 mRNA(NF-κB p 65 mRNA),IκB mRNA,interleukin-1β(IL-1β )and tumor necrosis factor-α(TNF-α)in rats with cerebral ischemia(CI),so as to investigate its molecular mechanisms underlying improving CI by reducing inflammatory response.Methods A total of 64 SD rats were randomized into normal control,model,medication and scalp-acupuncture groups,with 16 rats in each group.The focal CI model was established by middle cerebral artery occlusion(MCAO).Intraperitoneal injection of Pyrrolidine Dithiocarbamate(100 mg·kg-1·d-1)was administrated for rats in the medication group,once a day for 7 days.For rats of the scalp-acupuncture group,the acupuncture needles were rapidly inserted into bilateral Dingnieqianxiexian(MS 6)and Dingniehouxiexian(MS 7),followed by twirling the needles at 200 cycles/min for 1 min,once again every 10 min during 30 min's needle retention.The treatment was conducted once a day for 7 days.The neurologic deficit score(0-5 points,impaired consciousness,death,etc.)and neurological function score(motor,sensory and sensory tests,0-10 points)were assessed according to Longa's(1989)and Schbitz's(2004)methods,respectively.The expression levels of NF-κB p 65 mRNA and IκB mRNA in the parahippocampus gyrus tissue were detected by fluorescence quantitative reverse transcription-PCR,and IL-1β and TNF-αproteins in the parahippocampus gyrus tissue were detected by immunohistochemistry.Results After modeling,the neurologic deficit and neurological function scores and the expression levels of NF-κB p 65 mRNA,IL-1β and TNF-αin the parahippocampus were significantly increased in the model group than in the normal group(P<0.01),while the expression of IκB mRNA was considerably down-regulated(P<0.01).Following treatment intervention,the neurologic deficit and neurological function scores as well as NF-κB p 65 mRNA,and IL-1β and TNF-αprotein expression were significantly decreased in both scalp-acupuncture and medication groups compared with the model group(P<0.05,P<0.01),and the decreased expression of IκB mRNA was obviously increased(P<0.05).Conclusion Scalp-acupuncture can improve neurologic function in cerebral ischemic rats,which is related with its effects in up-regulating the expression of IκB to inhibit the dissociation of NF-κB,then decreasing the expression of IL-1β and TNF-α(reducing inflammatory response)in the parahippocampal gyrus tissue.

关键词(KeyWords)： 头针;急性缺血性脑血管病;神经功能缺损;海马旁回;核因子-κB p 65 mRNA;IκB mRNA;白介素-1β;肿瘤坏死因子-α
Scalp acupuncture;Acute cerebral ischemia;Neurologic deficit;Parahippocampus gyrus;NF-κB p 65 mRNA;IκB mRNA;Interleukin-1β(IL-1β);Tumor necrosis factor-α(TNF-α)

Abstract:

Keywords:

基金项目(Foundation): 兰州大学第二医院院内博士科研基金(No.2015-1-26)

作者(Authors): 王金海;张婷卓;赵敏;鲍英存;鄢琦;张振昶;杜小正;姜华;田亮;袁博;李兴兰;
WANG Jin-hai;ZHANG Ting-zhuo;ZHAO Min;BAO Ying-cun;YAN Qi;ZHANG Zhen-chang;DU Xiao-zheng;JIANG Hua;TIAN Liang;YUAN Bo;LI Xing-lan;Department of Traditional Chinese Medicine,Second Hospital of Lanzhou University;School of Acupuncture-moxibustion and Massage,Gansu University of Traditional Chinese Medicine;Department of Neurology,Second Hospital of Lanzhou University;

DOI: 10.13702/j.1000-0607.170532

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