针刺研究

2021, v.46(09) 775-781

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

电针抑制NF-κB活性改善慢性疲劳综合征大鼠认知功能障碍的实验研究
Electroacupuncture improves cognitive function by inhibiting NF-κB activity in rats with chronic fatigue syndrome

冯楚文;屈媛媛;孙忠人;王玉琳;张莑;王庆勇;林婉娟;张琳;杨添淞;
FENG Chu-wen;QU Yuan-yuan;SUN Zhong-ren;WANG Yu-lin;ZHANG Peng;WANG Qing-yong;LIN Wan-juan;ZHANG Lin;YANG Tian-song;First Affiliated Hospital, 2Graduate School, Heilongjiang University of Chinese Medicine;Second Affiliated Hospital of Heilongjiang University of Chinese Medicine;Shenzhen Hospital of Beijing University of Chinese Medicine in Longgang;Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University;

摘要(Abstract):

目的:观察电针对慢性疲劳综合征(CFS)大鼠海马核转录因子κB p65(NF-κB p65)表达水平及海马组织形态的影响,探讨电针治疗CFS大鼠认知功能障碍的作用机制。方法:从SD大鼠中随机抽取12只作为空白组,36只采用多因素复合应激刺激法建立CFS模型,造模成功后随机分为模型组、抑制剂组、电针组,每组12只。造模后,电针组电针"百会"、情感Ⅰ区、感觉区(双侧),每日1次,30 min/次,共15 d。抑制剂组采用吡咯烷二硫基甲酸盐100 mg·kg~(-1)·d~(-1)腹腔注射,每日1次,共15 d。以Morris水迷宫实验评价大鼠学习记忆能力;HE染色法观察海马组织形态;Western blot检测海马组织NF-κB p65的表达水平。结果:与空白组比较,模型组一般情况评分明显升高(P<0.01),水迷宫实验潜伏期延长(P<0.01),穿越平台次数减少(P<0.01),海马组织NF-κB p65表达升高(P<0.05);与模型组比较,抑制剂组和电针组一般情况评分明显降低(P<0.01),水迷宫实验潜伏期缩短(P<0.01),穿越平台次数增多(P<0.01),NF-κB p65表达下降(P<0.05)。HE染色结果显示,与空白组比较,模型组海马区神经细胞排列紊乱,结构疏松,凋亡小体和炎性细胞数量明显增多;与模型组比较,抑制剂组和电针组海马区神经细胞排列较规整,结构较紧密,细胞形态有一定的改善,凋亡小体和炎性细胞数量减少。结论:电针可能通过抑制NF-κB的激活,减轻海马组织炎性浸润,从而缓解CFS大鼠疲劳状态,提高大鼠学习、记忆能力,改善认知功能障碍。
Objective To observe the effect of electroacupuncture(EA) on the expression of NF-κB p65 in hippocampus and the morphology of hippocampus in rats with chronic fatigue syndrome(CFS), so as to explore its mechanism in improving cognitive dysfunction of CFS. Methods Forty-eight SD rats were randomly divided into control, model, EA and inhibitor groups(n=12 in each group). The CFS model was established by multi-factor compound stress stimulation method. Rats of the EA group received EA(50 Hz, 1 mA) at "Baihui"(GV20), Emotional Area I and bilateral Sensory Area for 30 min, once daily for 15 days. For rats in the inhibitor group, pyrrolidine dithiocarbamate(100 mg·kg~(-1)·d~(-1)) was injected intraperitoneally, once a day for 15 days. Learning and memory ability was evaluated by Morris water maze test. HE staining was used to observe the morphology of hippocampus. Western blot was used to determine the expression level of NF-κB p65 in hippocampus. Results After mode-ling, the general status score was increased(P<0.01), the escape latency was prolonged(P<0.01), the times of crossing the platform was decreased(P<0.01), and the expression level of NF-κB p65 in hippocampus tissue was significantly increased(P<0.05) in the model group compared with the control group. Compared with the model group, the general status score was decreased(P<0.01), the escape latency was shortened(P<0.01), the times of crossing the platform was increased(P<0.01), and the expression level of NF-κB p65 in hippocampus tissue was significantly decreased(P<0.05) in the EA and inhibitor groups. HE staining showed that in the model group, the hippocampal nerve cells were arranged disorderly, the structure was loose, and the number of apoptotic bodies and inflammatory cells was significantly increased. The degree of tissue damage of the EA and inhibitor groups was milder than that of the model group. Conclusion EA can improve the cognitive function in CFS rats, which may be associated with its effect in inhibiting the expression of NF-κB and reducing the inflammation response in hippocampus.

关键词(KeyWords): 电针;核转录因子κB;慢性疲劳综合征;实验研究
Electroacupuncture;NF-κB;Chronic fatigue syndrome;Experimental study

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.81704170、82074539);; 黑龙江省自然科学基金项目(No.LH2020H092);; 黑龙江省博士后启动金项目(No.LBH-Q18117);; 深圳市人民医院中青年科研技术骨干培育项目(No.SYKYPY201925)

作者(Author): 冯楚文;屈媛媛;孙忠人;王玉琳;张莑;王庆勇;林婉娟;张琳;杨添淞;
FENG Chu-wen;QU Yuan-yuan;SUN Zhong-ren;WANG Yu-lin;ZHANG Peng;WANG Qing-yong;LIN Wan-juan;ZHANG Lin;YANG Tian-song;First Affiliated Hospital, 2Graduate School, Heilongjiang University of Chinese Medicine;Second Affiliated Hospital of Heilongjiang University of Chinese Medicine;Shenzhen Hospital of Beijing University of Chinese Medicine in Longgang;Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University;

Email:

DOI: 10.13702/j.1000-0607.200827

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享