针刺研究

2012, v.37(02) 114-118

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火针对类风湿性关节炎大鼠血清白介素-1和肿瘤坏死因子-α的影响
Effect of Fire-Needle Intervention on Serum IL-1 and TNF-α Levels of Rheumatoid Arthritis Rats

韩润霞;杨晶;张天生;张卫东;
HAN Run-xia,YANG Jing,ZHANG Tian-sheng1,ZHANG Wei-dong(Shanxi College of Chinese Medicine,Taiyuan 030024,China;1Shanxi Provincial Institute of Acu-moxibustion,Taiyuan 030006)

摘要(Abstract):

目的:观察火针对类风湿性关节炎大鼠血清白介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)的影响,探讨火针治疗类风湿性关节炎的作用机制。方法:将40只雌性Wistar大鼠随机分为正常组、模型组、甲氨蝶呤(MTX)组、火针组。于大鼠右后足跖底部皮内注射0.1mL完全弗氏佐剂建立类风湿性关节炎模型。火针点刺"夹脊穴""足三里"和"阿是穴",共治疗8次。容积法测量大鼠右后足肿胀度;双抗体夹心ABC-ELISA法检测大鼠血清IL-1和TNF-α含量;光镜观察大鼠膝关节滑膜组织的病理变化。结果:模型组大鼠足部肿胀度、血清IL-1和TNF-α的含量较正常组显著升高(P<0.01);MTX组造模后33d足部肿胀度较模型组降低(P<0.01),火针组造模后19、26、33d足部肿胀度较模型组降低(P<0.01),治疗后MTX组和火针组大鼠血清IL-1和TNF-α的含量较模型组均明显降低(P<0.05,P<0.01),火针组与MTX组相比大鼠足部肿胀度、血清IL-1和TNF-α的含量明显降低(P<0.05,P<0.01)。与正常组比较,模型组大鼠滑膜组织有明显的病理改变,各治疗组均能改善膝关节滑膜组织的炎性病变,且火针对滑膜病变的改善优于MTX。结论:火针可降低类风湿性关节炎大鼠血清IL-1和TNF-α的含量,减轻关节局部肿胀度,改善滑膜病变,有效控制类风湿性关节炎的病情发展。
Objective To observe the effect of fire-needle intervention on serum IL-1 and TNF-α contents in rheumatoid arthritis(RA) rats so as to study its underlying mechanism in improving RA.Methods A total of 40 Wistar rats were randomized into normal control,model,methotrexate(MTX,medication) group and fire-needle groups,with 10 cases in each group.RA model was established by injecting Freund's complete adjuvant(0.1 mL) into the rats' right hind paw.Fire-needle was applied to"Jiaji"(EX-B 2),"Zusanli"(ST 36) and "Ashi point"(the most severe site of the swelling joint),once every three days and 8 times altogether.The rat's right hindpaw swelling volume was detected by volumetric method,and serum IL-1 and TNF-α contents were determined by double antibody sandwich ELISA.The pathological changes of synovium tissue of the right knee-joint were observed by optical microscope.Results In comparison with the control group,the swollen paw volumes at time-points of 12 d,19 d,26 d and 33 d after modeling and serum IL-1 and TNF-α contents were increased significantly(P<0.01).Compared to the model group,the swollen paw volumes on day 33 in both medication and fire-needle groups were decreased considerably(P<0.01),while serum IL-1 and TNF-α contents in the medication and fire-needle groups were reduced significantly(P<0.05,P<0.01).No significant differences were found between the medication and fire-needle groups in the swollen paw volumes on day 12(P>0.05).The pathological states(synovial cells' arrangement,inflammatory infiltration,synovial membrane thicke-ning,etc.) of synovium tissue of the right knee-joint in both medication and fire-needle groups were relatively lighter than those of the model group.Conclusion Fire-needle therapy can down-regulate serum IL-1 and TNF-α contents in rheumatoid arthritis rats,and reduce the degree of arthroncus of the affected joint,which may contribute to its effect in improving synovial lesions.

关键词(KeyWords): 类风湿性关节炎;火针;药物;IL-1;TNF-α
Rheumatoid arthritis;Fire-needle therapy;Medication;Serum IL-1;TNF-α

Abstract:

Keywords:

基金项目(Foundation): 山西省自然科学基金资助(2008011083)

作者(Author): 韩润霞;杨晶;张天生;张卫东;
HAN Run-xia,YANG Jing,ZHANG Tian-sheng1,ZHANG Wei-dong(Shanxi College of Chinese Medicine,Taiyuan 030024,China;1Shanxi Provincial Institute of Acu-moxibustion,Taiyuan 030006)

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DOI: 10.13702/j.1000-0607.2012.02.008

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